Color recognition using Bacteriorhodopsin with synthetic retinal amalogues

使用细菌视紫红质和合成视网膜类似物进行颜色识别

• 批准号: 10650004
• 负责人: OKADA Yoshiko
• 金额: $ 1.86万
• 依托单位: The University of Electro-Communications
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10650004/
• 关键词: Bacteriorhodopsin; visual pigment; retinal amalogue; blue membrane; color recognition; color sensor; 青膜; 視物質

Bacteriorhodopsin (bR) is the best understood protein of the class of rhodopsins light sensitive pigments. Most known member of that family is rhodopsin, protein responsible for the light detection in human eye. We proposed a color photosensor made of bR and its variants.We simulated basic nonlinear optical proerties. (refrectivity and absorption) of wild type bacteriorhodopsin for green or red sensitive cone visual pigment. A variant of wild type bR is prepared by reconstituting bleached bR with 4-keto retinal as green sensitive cone. Absorption maxima for bR-4-keto is 515 nm, but the shape of spectrum is not sharp. Wt-bR can be changed to a blue species by acid (HC1) washing. Absorption spectra of blue membrane by our treatments shifted blue(λィイD2maxィエD2 = 600 nm). Tomioka isolated a phoborhodopsin which absorption maxima is 410 nm (or 490 nm). From the above results, we proposed artificial retina which have blue membrane, wt-bR and phoborhodopsin in place of red, green and blue sensitive cone visual pigments, respectivery.

细菌视紫红质（bR）是一类被了解最多的视紫红质感光色素。该家族中最著名的成员是视紫红质，一种负责人眼光探测的蛋白质。我们提出了一种由bR及其变体制成的彩色光敏器。我们模拟了基本的非线性光学性质。（反射率和吸收）野生型细菌视紫红质为绿色或红色敏感的视锥色素。以4-酮视网膜为绿色敏感锥体，将脱色后的bR重组为野生型bR。br -4-酮的吸光度最大值为515 nm，但光谱形状不清晰。Wt-bR经酸（HC1）洗涤可变为蓝色。处理后的蓝膜吸收光谱发生蓝移（λ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ）。Tomioka分离出一种最大吸收为410 nm（或490 nm）的光紫红质。根据以上结果，我们提出了用蓝膜、wt-bR和磷视紫红质分别代替红、绿、蓝敏感视锥色素的人工视网膜。

项目成果

Y. Okamoto, T. Fujiki, Y. Okada-Shudo and I. Yamaguchi: "Nimerical analysis of real-time holography in bacteriorhodopsin films"Opt. Eng.. 38(1). 157-163 (1999)

Y. Okamoto、T. Fujiki、Y. Okada-Shudo 和 I. Yamaguchi：“细菌视紫红质薄膜中实时全息的数值分析”Opt。

S. Ashihara et al: "Autocorrelation of picosecond pulses in bacteriorhodopsin film using light self-diffraction form intensity and polarization"Opt. Commun.. 165. 83-89 (1999)

S. Ashihara 等人：“利用光自衍射形式强度和偏振对细菌视紫红质膜中的皮秒脉冲进行自相关”Opt。

S. Ashihara, K. Kuroda, Y. Okada-Shudo, and K. Jarasiunas: "Autocorrelation of picosecond pulses in bacteriorhodopsin film using light self-diffraction from intensity and polarization"Opt. Commun.. 165. 83-89 (1999)

S. Ashihara、K. Kuroda、Y. Okada-Shudo 和 K. Jarasiunas：“利用来自强度和偏振的光自衍射对细菌视紫红质膜中的皮秒脉冲进行自相关”Opt。

Y. Okada-Shudo: "Polarization recording and reconstruction in a Photoinduced Anisotropic Medium"Nonlinear Optics. (in press). (2000)

Y. Okada-Shudo：“光致各向异性介质中的偏振记录和重建”非线性光学。

M.Kamekura et al.: "Detection and expression of a gene encoding a new bacteriorhodopsin from an extreme halophile strain HT(JCM9743) which does not possess bacteriorhodopsin activity"Extremophiles. 2. 33-39 (1998)

M.Kamekura 等人：“从不具有细菌视紫红质活性的极端嗜盐菌菌株 HT（JCM9743）中检测和表达编码新细菌视紫红质的基因”极端微生物。