Carbon conversion in mixotrophic cultivation by a thermophilic cyanobaterium producing PHB

产生 PHB 的嗜热氰藻在混合营养培养中的碳转化

基本信息

  • 批准号:
    10650785
  • 负责人:
  • 金额:
    $ 2.56万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    1998
  • 资助国家:
    日本
  • 起止时间:
    1998 至 1999
  • 项目状态:
    已结题

项目摘要

In the present research, the utilization properties of carbon and energy sources were characterized in the photomixotrophic cultures of a thermophilic cyanobacterium (Synechococcus sp.MA19) and liverwort cells (Marchantia polymorpha HYA-2F), and the following results were obtained.(1) Sucrose was selected as an organic carbon source which was utilized by strain MA19 under a photomixotrophic condition. It was found that phosphate was a key factor governing the productivity of poly-β-hydroxybutyrate (PHB) by the strain.(2) In the cultures of stratn MA19 with sucrose medium in the range of incident light intensity of 0-67 W/m^2, the cellular carbon recovery of 1.1 and CO_2 evolution ratio of -0.18 were obtained when the total light energy absorbed by the cells was 5.8×10^6 J/g-cells. This result inducated that CO_2 released via respiratory actions was trapped and reused for the cell formation by photosynthetic activity of the strain.(3) A bioreactor system was developed for the photomixotrophic cultures of M.polymorpha HYA-2F, which consisted of a glass vessel with external light irradiation and a light moderator installed in a process controller. Batch culture in the bioreactor was conducted in glucose medium while keeping the value of absorbed light energy at 3.5×10^2 W/kg by regulating incident light intensity ranging from 19 to 220 W/m^2. During this culture under light control, CO_2 evolution was suppressed and the high value of cell yield was achieved compared with that in the culture conducted at constant incident light intensity of 50 W/m^2.(4) The characteristics of energy conversion through respiratory and photosynthetic actions in the mixotrophic cells were examined based on the metabolic flux analysis. In the culture with light regulation where the efficiency of overall energy conversion to ATP was considered, the enhanced cell yield was obtained on the basis of energy provided to the system in comparison with those in the cultures without light control.
在本研究中,碳和能源的利用特性的光混合营养培养的嗜热蓝细菌(聚球藻属MA 19)和地钱细胞(地钱HYA-2F)的特点,并获得了以下结果。(1)以蔗糖为有机碳源,在光混合营养条件下对菌株MA 19进行了研究。结果表明,磷酸盐是影响该菌株产聚β-羟基丁酸酯(PHB)的关键因素。(2)在入射光强为0-67 W/m^2的蔗糖培养基中,当细胞吸收的总光能为5.8× 10^6J/g细胞时,细胞碳回收率为1.1,CO_2释放率为-0.18。这一结果表明,呼吸作用释放的CO_2被光合作用捕获并重新用于细胞形成。(3)建立了一种多形分枝杆菌HYA-2F光混合营养培养的生物反应器系统,该系统由一个玻璃容器和一个安装在过程控制器中的光调节器组成。在葡萄糖培养基中进行生物反应器中的分批培养,同时通过调节入射光强度在19至220 W/m^2范围内来保持吸收光能值为3.5×10^2 W/kg。与恒定入射光强50 W/m^2培养相比,在光控制下培养,CO_2释放受到抑制,细胞产量达到较高值。(4)通过代谢通量分析,研究了兼养细胞呼吸和光合作用的能量转换特性。在考虑总能量转化为ATP的效率的具有光调节的培养物中,与没有光控制的培养物相比,基于提供给系统的能量获得了增强的细胞产率。

项目成果

期刊论文数量(21)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Hata J.et al.: "Utilization property of organic carbon source in photomixotrophic culture of liverwort cells"Proc.the 5th Asia-Pacific Biochem.Eng.. 306 (1999)
Hata J.等:“地钱细胞光合营养培养物中有机碳源的利用特性”Proc.the 5th Asia-Pacific Biochem.Eng.. 306 (1999)
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    0
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  • 通讯作者:
福井ら: "好熱性シアノバクテリアの有機炭素源資化性"化学工学会徳島大会(平成11年度)講演要旨集. 136-137 (1999)
Fukui 等人:“嗜热蓝藻的有机碳源利用”化学工程师协会德岛会议记录(1999 年)136-137(1999 年)。
  • DOI:
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    0
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  • 通讯作者:
Hata, J.et al.: "Utilization property of organic carbon source in photomixotrophic culture of liverwort cells"Proc.the 5th Asia-Pacific Biochem.Eng.. 306 (1999)
Hata,J.et al.:“地钱细胞光合营养培养中有机碳源的利用特性”Proc.the 5th Asia-Pacific Biochem.Eng.. 306 (1999)
  • DOI:
  • 发表时间:
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  • 影响因子:
    0
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  • 通讯作者:
Hata, J.et al.: "Evaluation of carbohydrate utilization and photosysnthetic carbon dioxide fixation in photomixotrophic culture of Marchantia polymorpha"J.Chem.Eng.Japan.. 33-2. 277-284 (2000)
Hata,J.等人:“地钱光合营养培养物中碳水化合物利用和光合二氧化碳固定的评估”J.Chem.Eng.Japan. 33-2。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Hata J.et al.: "Reduction in carbon dioxide emission, and enhancement of cell yield by control of light intensity in photomixotrophic batch culture of Marchantia polymorpha"J.Biosci.Bioeng.. 89巻3号(印刷中). (2000)
Hata J. 等人:“通过控制地钱光合营养分批培养中的光强度减少二氧化碳排放并提高细胞产量”J.Biosci.Bioeng.. 第 89 卷,第 3 期(出版中) (2000)
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TAYA Masahito其他文献

TAYA Masahito的其他文献

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{{ truncateString('TAYA Masahito', 18)}}的其他基金

Formulation of parameters for quantitative definition of the vitality of human cells and evaluation of their life-span
制定定量定义人体细胞活力和评估其寿命的参数
  • 批准号:
    22360344
  • 财政年份:
    2010
  • 资助金额:
    $ 2.56万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
LONG-TERM CONSERVATION AND EFFECTIVE REGENERATION OF PLANT CELLS IN ARTIFICIAL SEED CAPSULES
人造种子胶囊中植物细胞的长期保存和有效再生
  • 批准号:
    08650943
  • 财政年份:
    1996
  • 资助金额:
    $ 2.56万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
DERIVATION OF HAIRY ROOTS EXPRESSING BIOLOGICAL REACTIONS IN LEAF TISSUE AND PRODUCTION OF USEFUL MATERIALS IN CULTURE WITH PHOTOBIOREACTOR
利用光生物反应器衍生表达叶组织生物反应的毛状根并生产有用的培养材料
  • 批准号:
    06650915
  • 财政年份:
    1994
  • 资助金额:
    $ 2.56万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
Development of Sterilization Strategy for Thermoduric Bacterial Spores in Photoreaction System with Solar Source
太阳能光反应系统中耐高温细菌孢子灭菌策略的研究
  • 批准号:
    05555222
  • 财政年份:
    1993
  • 资助金额:
    $ 2.56万
  • 项目类别:
    Grant-in-Aid for Developmental Scientific Research (B)
Production of Valuable Metabolites by Plant Hairy Root Cultures and Establishment of System to Harvest the Products
植物毛状根培养物生产有价值的代谢物及产品收获系统的建立
  • 批准号:
    01550763
  • 财政年份:
    1989
  • 资助金额:
    $ 2.56万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)

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  • 批准号:
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Physiological role of novel UV-absorbing substances in the terrestrial cyanobacterium
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Collaborative Research: Dimensions: Integrating phylogenetics, ecophysiology and transcriptomics to understand the diversity of hornwort-cyanobacterium symbiosis
合作研究:维度:整合系统发育学、生态生理学和转录组学,了解金鱼藻-蓝藻共生的多样性
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