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DERIVATION OF HAIRY ROOTS EXPRESSING BIOLOGICAL REACTIONS IN LEAF TISSUE AND PRODUCTION OF USEFUL MATERIALS IN CULTURE WITH PHOTOBIOREACTOR

利用光生物反应器衍生表达叶组织生物反应的毛状根并生产有用的培养材料

基本信息

批准号：
06650915
负责人：
TAYA Masahito
金额：
$ 1.22万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

1.Hairy roots of pak-bung turned green when cultured under continuous light exposure, and maintained their branched root morphology with the formation of chlorophyll and the development of a thylakoid membrane system in the cells. Green hairy roots cultivated under light exhibited high root growth and enhanced activities of superoxide dismutase (SOD) and peroxidase (POD). In cultures of green hairy roots at a light intensity of 11.1 W/m^2, root growth, total SOD activity and total POD activity obtained on culture day 21 were 8.1g-dry root weight/dm^3,18x10^4 U/dm^3 and 40x10^3 U/dm^3, respectively. In dark culture, the corresponding values obtained on culture day 21 were 3.8 g-dry root weight/dm^3,3.0x10^4 U/dm^3 and 9.5x10^3 U/dm^3, respectively.2.Under the light condition, the growth was promoted accompanying the increase in number of growing points of roots. Concerning the chlorophyll formation in the hairy roots exposed to light, chlorophyll content increased with the increase in distance from the root tips and gradually approached a saturated value. This positional dependence of chlorophyll content was considered to arise from the distribution in cellular age due to a linear growth of the hairy roots. Based on these results, a kinetic model of chlorophyll formation associated with the growth was presented. The calculated values of cell mass and chlorophyll content were in fair agreement with experimental ones, indicating that the model made it possible to describe the kinetic behaviors of growth and chlorophyll formation in the hairy root cultures.3.Green hairy roots could be cultivated in an internally illuminated bioreactor. It was observed that the chlorophyll content, and SOD and POD activities in the cells changed depending on the location of the hairy roots because of the distribution of light intensity in the reactor.

1.在连续光照条件下培养的白峰毛状根变绿色，并通过叶绿素的形成和细胞内类囊体膜系统的发育维持其分枝根的形态。光照下培养的绿色毛状根具有较高的根系生长量和较高的超氧化物歧化酶（SOD）和过氧化物酶（POD）活性。绿色毛状根在11.1 W/m^2的光照强度下培养21 d，其根生长量、总SOD活性和总POD活性分别为8.1 g-干根重/dm^3、18 × 10^4 U/dm^3和40 × 10^3 U/dm^3。在暗培养条件下，培养第21天的相应值分别为3.8 g-干根重/dm^3、3.0 × 10^4 U/dm^3和9.5 × 10^3 U/dm^3。在光照条件下，毛状根中叶绿素含量随着离根尖距离的增加而增加，并逐渐接近饱和值。叶绿素含量的这种位置依赖性被认为是由于毛状根的线性生长而引起的细胞年龄分布。在此基础上，提出了与生长相关的叶绿素形成动力学模型。细胞质量和叶绿素含量的计算值与实验值吻合较好，表明该模型能够较好地描述毛状根培养过程中的生长和叶绿素生成动力学行为。结果表明，由于反应器内光照强度的分布，毛状根所在位置不同，细胞内叶绿素含量、SOD和POD活性也不同。