DERIVATION OF HAIRY ROOTS EXPRESSING BIOLOGICAL REACTIONS IN LEAF TISSUE AND PRODUCTION OF USEFUL MATERIALS IN CULTURE WITH PHOTOBIOREACTOR

1.白蓬毛状根在连续光照下呈绿色，随着细胞中叶绿素的形成和类囊体膜系统的发育，毛状根的形态保持不变。绿毛状根在光照条件下生长旺盛，超氧化物歧化酶和过氧化物酶活性增强。在光强为11.1W/m^2的绿色毛状根培养中，培养第21天的根生长、总SOD活性和总POD活性分别为8.1g/dm~3、18×10~(-4)U/dm^3和40×10~(-3)U/dm^3。暗培养第21天的根干重分别为3.8g/dm^3、3.0×10~(-4)U/dm^3和9.5×10~(-3)U/dm^3。从毛状根的叶绿素形成来看，随着离根尖距离的增加，毛状根的叶绿素含量逐渐增加，并逐渐趋于饱和。这种对叶绿素含量的位置依赖性被认为是由于毛状根的线性生长引起的细胞年龄的分布。在此基础上，提出了一个与生长相关的叶绿素形成动力学模型。细胞质量和叶绿素含量的计算值与实验值吻合较好，表明该模型能够描述毛状根培养过程中生长和叶绿素形成的动力学行为。3.绿色毛状根可以在内光生物反应器中培养。由于反应器内光强的分布，毛状根的位置不同，细胞内的叶绿素含量、超氧化物歧化酶和过氧化物酶活性也会发生变化。

1.Hairy roots of pak-bung turned green when cultured under continuous light exposure, and maintained their branched root morphology with the formation of chlorophyll and the development of a thylakoid membrane system in the cells. Green hairy roots cultivated under light exhibited high root growth and enhanced activities of superoxide dismutase (SOD) and peroxidase (POD). In cultures of green hairy roots at a light intensity of 11.1 W/m^2, root growth, total SOD activity and total POD activity obtained on culture day 21 were 8.1g-dry root weight/dm^3,18x10^4 U/dm^3 and 40x10^3 U/dm^3, respectively. In dark culture, the corresponding values obtained on culture day 21 were 3.8 g-dry root weight/dm^3,3.0x10^4 U/dm^3 and 9.5x10^3 U/dm^3, respectively.2.Under the light condition, the growth was promoted accompanying the increase in number of growing points of roots. Concerning the chlorophyll formation in the hairy roots exposed to light, chlorophyll content increased with the increase in distance from the root tips and gradually approached a saturated value. This positional dependence of chlorophyll content was considered to arise from the distribution in cellular age due to a linear growth of the hairy roots. Based on these results, a kinetic model of chlorophyll formation associated with the growth was presented. The calculated values of cell mass and chlorophyll content were in fair agreement with experimental ones, indicating that the model made it possible to describe the kinetic behaviors of growth and chlorophyll formation in the hairy root cultures.3.Green hairy roots could be cultivated in an internally illuminated bioreactor. It was observed that the chlorophyll content, and SOD and POD activities in the cells changed depending on the location of the hairy roots because of the distribution of light intensity in the reactor.