Formulation of parameters for quantitative definition of the vitality of human cells and evaluation of their life-span

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We formulated parameters for defining quantitatively the vitality of human cells and evaluated their life-span. First, passage cultures of human keratinocytes showed that the addition of hydrogen peroxide shortened the life-span of human keratinocytes. The drastic increases of average cell area and gene expression were observed when the remaining number of population doublings reached a specified value. Next, we focused on the megakaryocytic(MK) differentiation of human cells, which is the development of polyploidy cells via endomitosis. We revealed that the progression of committed MK cells to the endomitotic phase of differentiation depended on the reactive oxygen species (ROS) level. These results provide an evidence showing the importance of oxidative stress to MK differentiation.