Elucidation of immnoregulating function of casein phosphopeptide
酪蛋白磷酸肽免疫调节功能的阐明
基本信息
- 批准号:10660258
- 负责人:
- 金额:$ 2.3万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:1998
- 资助国家:日本
- 起止时间:1998 至 2000
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
An immunomodulating peptide was purified from a trypsin digest of bovine αs1-casein and identified as residues 59-79 in bovine αs1-casein. The sequence 59-79 has a Phosphoserine-rich region and is called one of casein phosphopeptides. Bovine β-casein aloso possesses phosphoserin-rich region. Hence, the author isolated β-casein fragment 1-25, one of the casein phosphopeptides, from tryptic β-casein and confirmed that the phosphopeptide displayed the immunomodulating action as well as the casein phosphopeptide from bovine αs1-casein.The casein phosphopeptides displayed a mitogenic activity and stimulated the mitogen-induced proliferations and the immunoglobulin production via the stimulation of IL-5 and IL-6 produced from Th2 cells in mouse spleen cell cultures. The oral adminstration of casein phosphopeptides enhanced the levels of antigen-specific IgA and total IgA in mice and piglets. Moreover, the immunostimulating activity was confirmed to a phosphopeptide having at least 2 phosphoserine residues, i.e., SerP-Leu-SerP.It has been widely reported that various kinds of casein phosphopeptides are produced from bovine caseins by the action of various kinds of proteolytic enzymes. Thus, the casein phosphopeptides prepared from bovine casein digests are beneficial foodstuffs for the enhancement of mucosal IgA responses in man and livestock.
从牛αs1-酪蛋白的胰蛋白酶消化中纯化了一种免疫调节肽,鉴定为牛αs1-酪蛋白残基59 ~ 79。序列59-79有一个富含磷酸丝氨酸的区域,被称为酪蛋白磷酸肽之一。牛β-酪蛋白也具有富磷丝蛋白区。因此,作者从胰蛋白酶β-酪蛋白中分离出酪蛋白磷酸化肽之一β-酪蛋白片段1-25,证实该肽与牛α 51 -酪蛋白中的酪蛋白磷酸化肽一样具有免疫调节作用。酪蛋白磷脂肽显示有丝分裂活性,并通过刺激小鼠脾细胞培养中Th2细胞产生的IL-5和IL-6,刺激有丝分裂原诱导的增殖和免疫球蛋白的产生。口服酪蛋白磷酸肽可提高小鼠和仔猪抗原特异性IgA和总IgA水平。此外,免疫刺激活性被证实为具有至少2个磷酸丝氨酸残基的磷酸肽,即SerP-Leu-SerP。牛酪蛋白在多种蛋白水解酶的作用下可合成多种酪蛋白磷酸肽。因此,从牛酪蛋白消化液中制备的酪蛋白磷酸肽是增强人和牲畜粘膜IgA反应的有益食品。
项目成果
期刊论文数量(32)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Hajime Otani: "Current Research of Advanced Series in Agricultural & Biological Chemistry"World Wide Research Network Publisher(in press). (2001)
Hajime Otani:“农业先进系列研究现状
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- 影响因子:0
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- 通讯作者:
Hajime Otani, Yukihiro Kihara and Minkyu Park: "The immunoenhancing property of a dietary casein phosphopeptide preparation in mice"Food and Agricultural Immunology. 12. 165-173 (2000)
Hajime Otani、Yukihiro Kihara 和 Minkyu Park:“小鼠膳食酪蛋白磷酸肽制剂的免疫增强特性”食品和农业免疫学。
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- 影响因子:0
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M. A. Matin & H. Otani: "Release of cytotoxic glycopeptides from human acid casein fraction by the action of stomach proteinases"Milchwissenschaft. 55(1). 6-10 (2000)
M.A.马丁
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I.Hata,S.Higashiyama & H.Otani: "Identification of a phosphopeptide in bovine αs1-casein digest as a factor influencing proliferation and immunogloblin production in lymphocyte cultures"Journal of Dairy Research. 65. 569-578 (1998)
I.Hata、S.Higashiyama 和 H.Otani:“牛 αs1-酪蛋白消化物中磷酸肽作为影响淋巴细胞培养物增殖和免疫球蛋白产生的因素的鉴定”《乳制品研究杂志》65. 569-578 (1998)。
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- 影响因子:0
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H.Otani,H.Kitamura et al.,: "Enhancement of intestinal IgA levels in piglets by oral administration of a commercially available casein phosphopeptide preparation"Milchwissenschaft. 55. 429-432 (2000)
H.Otani、H.Kitamura 等人:“通过口服市售酪蛋白磷酸肽制剂提高仔猪肠道 IgA 水平”Milchwissenschaft。
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OTANI Hajime其他文献
OTANI Hajime的其他文献
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心肌细胞特异性心肌保存
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具有粘膜免疫刺激能力的非过敏性肽制剂的研制
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12671335 - 财政年份:2000
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Myocardial protection during heart surgery by IGF-1 and bcl-2 gene introduction.
通过引入 IGF-1 和 bcl-2 基因来保护心脏手术期间的心肌。
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10671275 - 财政年份:1998
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Na^+/H^+ Exchange Plays a Role in Hypoxia-Reoxygenation induced ICAM-1 expression in CME
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Immunosuppresive action of milk k-casein and its digests
牛奶酪蛋白及其消化物的免疫抑制作用
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08660328 - 财政年份:1996
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相似海外基金
Potential of casein phosphopeptides to prevent tooth de cay
酪蛋白磷酸肽预防蛀牙的潜力
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nhmrc : 920816 - 财政年份:1992
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