Studies on anti-allergic properties of a mixture of Saccaromyces pastorianus and its specific cow's milk antibody

巴斯德酵母混合物及其特异性牛奶抗体抗过敏特性的研究

• 批准号: 22580306
• 负责人: OTANI Hajime
• 金额: $ 2.83万
• 依托单位: Shinshu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22580306/
• 关键词: 牛乳抗体; アレルギー軽減; 機能性食品素材; ビール酵母; 抗原抗体複合物; I 型アレルギー軽減; マウス毒性; 乳利用; 安全性; 食品; 牛乳IgG; 抗原・IgG複合体; I型アレルギー軽減; マウス; 脱顆粒; 畜産学; 免疫学; アレルギー・ぜんそく; たんぱく質; ビール酵母・IgG混合物

The author prepared an IgG-rich fraction specific to Saccaromyces (S.) pastorianus (milk antibody) from colostrum produced by a S. pastorianus-immunized cow. The allergic symptoms indicated as nose rubbing events of miceNC/Nga mice and ear thickness of BALB/c mice given a diet containing amixture of S. pastorianus and milk antibody (mixture-added diet) were significantly lower than in those given the S. pastorianus and milk antibody-free diet (control diet). The number of FcεRIα+CD117+ cells and mRNA expression of STIM1 were significantly lower in spleens of NC/Nga mice given the mixture-added diet than in those given the control diet. The mRNA expression of all subunit chains of IgE receptor, i.e. FcεRIα, FcεRIβ and FcεRIγ and proteins such as STIM1, BTK and ITK relating to degranulation was significantly reduced in C3H/HeN mouse spleen cell cultures with a mixture of S.pastorianus and milk antibody than in those without the S. pastorianus and/or milk antibody. On the other hand, only minimal acute toxicity was observed in ICR mice given 2,000 mg of S. pastorianusand/or milk antibody/g body weight. These results suggest that the mixture of S. pastorianus and milk antibody represents a safety anti-allergic food material.

作者制备了一种对囊酵母属（Saccaromyces（S.）pastorianus（乳抗体）来自由S. pastorianus免疫奶牛NC/Nga小鼠的鼻摩擦反应和BALB/c小鼠的耳增厚反应均表现为过敏症状。巴斯德酵母和牛奶抗体（混合添加饲料）显着低于那些给S。pastorianus和无乳抗体饮食（对照饮食）。与对照组相比，添加混合饲料的NC/Nga小鼠脾脏中FcεRIα+ CD 117+细胞数量和STIM 1 mRNA表达显著降低。在含有巴斯德毕赤酵母和乳抗体混合物的C3 H/HeN小鼠脾细胞培养物中，与不含巴斯德毕赤酵母的培养物相比，IgE受体所有亚基链（即FcεRIα、FcεRIβ和FcεRIγ）以及与脱颗粒相关的蛋白质（例如STIM 1、BTK和ITK）的mRNA表达显着降低。pastorianus和/或乳抗体。另一方面，在给予2，000 mg S的ICR小鼠中仅观察到极轻微的急性毒性。pastorianus和/或乳抗体/g体重。这些结果表明，S.巴斯德乳抗体代表了一种安全的抗过敏食品原料。

项目成果

パン酵母とそれに特異的なIgGを含むシバヤギ乳IgG分画のマウスI型アレルギー軽減作用

面包酵母和含有其特异性 IgG 的芝谷羊奶 IgG 级分的小鼠 I 型过敏降低作用

• 发表时间: 2011
• 期刊: New Food Industry
• 作者: Sekimura Y;Shimosato T and OtaniH;大谷元
• 通讯作者: 大谷元

The anti-allergic effects and acute toxicity of Lactobacillus crispatus KT-11 cultured in food grade medium

食品级培养基中卷曲乳杆菌KT-11的抗过敏作用和急性毒性

• 发表时间: 2010
• 期刊: Journal of Agriculture and Food Chemistry
• 作者: Tobita K;Yanaka H;Otani H
• 通讯作者: Otani H

Effect of Lactobacillus pentosus ONRIC b0240 on intestinal IgA production in mice fed differing levels of protein.

• DOI: 10.1021/jf104240d
• 发表时间: 2011-02
• 期刊: Journal of agricultural and food chemistry
• 影响因子: 6.1
• 作者: T. Shimosato;Kazuaki Tomida;H. Otani

Anti-allergic effects of a mixture of Saccharomyce cerevisiae and its specific goat's milk immunoglobulin G-rich fraction on ovalbumin-snecific BALB/c mice

酿酒酵母混合物及其富含羊奶免疫球蛋白 G 的混合物对卵清蛋白特异性 BALB/c 小鼠的抗过敏作用

• 发表时间: 2011
• 期刊: Milchwissenschaft
• 作者: Sekimura Y;Shimosato T;Otani H
• 通讯作者: Otani H

母乳の力～牛乳タンパク質に秘められた生体防御機能

母乳的力量：隐藏在乳蛋白中的生物防御功能

• 发表时间: 2011
• 作者: 河野俊夫;石川勝美;疋田慶夫;大谷元(単著)
• 通讯作者: 大谷元(単著)