The Mechanism of Tolerance against Hypothermia and Ischemia in Hibernators

冬眠者耐低温和缺血的机制

• 批准号: 12671335
• 负责人: OTANI Hajime
• 金额: $ 2.3万
• 依托单位: Kansai Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671335/
• 关键词: Hibernation; Hypothermia; Cardiac preservation; Left ventricular function; Myocardial infarction; Apoptosis; 虚血心筋

Objective : We have investigated the mechanism of tolerance against hypothermia and ischemia in deep hibernators in attempt to apply a hibernation phenomenon in cardiovascular surgery.Methods : Isolated hearts from hibernating and non-hibernating squirrels were rendered ischemia for 30 minutes at 37℃ or preserved in UW solution for 2 hours. Left ventricular function was evaluated by inflating the left ventricular balloon at a volume between 20-200 μL. Myocardial infarction was assessed by triphenyl-tetrazollum chloride staining. The number of apoptotic myocytes was calculated by a TUNEL method.Results : Left ventricular function, infarct size and the number of apoptotic myocytes were not significantly different between the hearts from hibernating and non-hibernating squirrels. In contrast, these parameters were significantly improved in the hearts from hibernating squirrels compared to non-hibernating squirrels.Conclusions : These results suggest that the hearts from hibernating squirrels are not signiflcantly tolerated to normothermic ischemia, but is resistant to myocyte deth during hypothermic preservation. Therefore, it is suggested that application of a hibernation phenomenon will be a promising approach in long term cardiac preservation.

目的：方法：将冬眠和非冬眠松鼠的离体心脏在37℃下缺血30分钟或UW液中保存2小时，观察冬眠动物对低温和缺血耐受的机制。通过以20-200 μL的体积充盈左心室球囊评价左心室功能。通过氯化三苯基四唑染色评估心肌梗死。结果：冬眠和非冬眠松鼠心脏的左室功能、心肌梗死面积和心肌细胞凋亡数无显著性差异。与此相反，这些参数显着改善冬眠松鼠的心脏相比，non-hibernating squirres.Conclusions：这些结果表明，冬眠松鼠的心脏是不是signiflantly耐受常温缺血，但耐低温保存过程中心肌细胞deth。因此，冬眠现象的应用将是一个有前途的长期保存心脏的方法。

项目成果

大谷 肇: "心臓手術と低体温療法"遺伝. 56. 68-73 (2002)

Hajime Otani：“心脏手术和低温治疗”遗传学 56. 68-73 (2002)。

Hajime Otani: "Cardiac Surgery and Hypothermic Therapy"Iden. 56. 68-73 (2002)

Hajime Otani：“心脏手术和低温治疗”Iden。