Myocardial regeneration by targeting to microRNA

通过靶向 microRNA 实现心肌再生

• 批准号: 23591070
• 负责人: OTANI Hajime
• 金额: $ 3.41万
• 依托单位: Kansai Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23591070/
• 关键词: 心筋再生; 心筋再生医療; マイクロRNA

We investigated whether controlled release of antagomir designed to inhibit microRNA-92a leads to enhanced blood vessel growth and functional recovery after myocardial infarction (MI) in rats. There was no difference in LV wall motion between rats with the antagomir-92a patch and the patch alone 1 hour after MI. However, myocardial thinning was inhibited, and LV wall motion was improved 3 days after MI in rats with the antagomir-92a patch. Myocardial fibrosis was reduced by the antagomir-92a patch 14 days after MI. Angiogenesis within the infarct area was increased by the antagomir-92a patch associated with enhanced cardiomyogenesis. The patch alone or the patch impregnated with antagomir-Co had no effect on LV wall motion, myocardial fibrosis, angiogenesis and cardiomyogenesis after MI. These results suggest that controlled release of antagomir-92a using gelatin hydrogel microspheres-incorporated patch is an effective tool for cardiac regeneration therapy after MI.

我们研究了旨在抑制microRNA-92 a的缓释舒洛米是否会增强大鼠心肌梗死（MI）后的血管生长和功能恢复。MI后1小时，贴敷Amplomir-92 a贴剂的大鼠和仅贴敷贴剂的大鼠之间的LV壁运动无差异。然而，在MI后3天，使用Escheromir-92 a贴剂的大鼠心肌变薄受到抑制，左室壁运动得到改善。心肌梗死后14天，Escheromir-92 a贴剂可减少心肌纤维化。梗死区域内的血管生成通过与增强的心肌生成相关的Escheromir-92 a贴剂而增加。单独的贴片或浸渍有Escheromir-Co的贴片对MI后的LV壁运动、心肌纤维化、血管生成和心肌形成没有影响。这些结果表明，使用明胶水凝胶微球掺入的贴剂控制释放的替吉奥-92 a是MI后心脏再生治疗的有效工具。

项目成果

Regeneration of the Heart by Controlled Release of Antagomir-92a Using Gelatin Hydrogel Microspheres-incorporated Patch in Rats with Myocardial Infarction

使用明胶水凝胶微球贴剂控制释放 Antagomir-92a 促进心肌梗塞大鼠心脏再生

• 发表时间: 2011
• 作者: Masanori Fujita;Hajime Otani;Takayuki Shimazu;Kei Yoshioka;Toru Okazaki;Daisuke Sato;Masayoshi Iwasaki;Toshiji Iwasaka;Yasuhiko Tabata
• 通讯作者: Yasuhiko Tabata

マイクロRNA92aを標的とした心筋再生医療

靶向microRNA92a的心肌再生医学

• 发表时间: 2013
• 作者: Teraguchi I;Imanishi T;Ozaki Y;Tanimoto T;Yamano T;Ino Y;Yamaguchi T;Hirata K;Kubo T;Akasaka T;大谷肇,藤田昌哲,岩崎真佳,藤高啓祐,塩島一朗,田端泰彦
• 通讯作者: 大谷肇,藤田昌哲,岩崎真佳,藤高啓祐,塩島一朗,田端泰彦

Antagomir-92a impregnated gelatin hydrogel microsphere sheet enhances cardiac regeneration after myocardial infarction in rats☆

Antagomir-92a浸渍明胶水凝胶微球片增强大鼠心肌梗死后心脏再生☆

• 发表时间: 2016
• 期刊: Regenerative Therapy
• 影响因子: 4.3
• 作者: M. Fujita;H. Otani;M. Iwasaki;K. Yoshioka;Takayuki Shimazu;I. Shiojima;Y. Tabata
• 通讯作者: Y. Tabata

Cardiomyocyte Regeneration after Myocardial Infarction through Upregulation of Integrin Alpha5

通过上调整合素 Alpha5 实现心肌梗死后心肌细胞再生

• 发表时间: 2012
• 作者: Masanori Fujita;Hajime Otani;Takayuki Shimazu;Kei Yoshioka;Toru Okazaki;Daisuke Sato;Masayoshi Iwasaki;Toshiji Iwasaka;Yasuhiko Tabata
• 通讯作者: Yasuhiko Tabata

Antagomir-92a Impregnated Gelatin Hydrogel Microsphere-incorporated patch Enhances Angiogenesis and Cardiomyocyte Regeneration after Myocardial Infarction through Upregulation of Integrin 5

Antagomir-92a 浸渍明胶水凝胶微球贴剂通过上调整合素增强心肌梗死后的血管生成和心肌细胞再生

• 发表时间: 2012
• 作者: 藤田正則;大谷 肇;嶋津孝幸;吉岡 慶;岩崎真佳;岩坂壽二;田端泰彦
• 通讯作者: 田端泰彦