The effect of stress on the plasma ACTH and cortisol concentrations after administration of Arginine-Vasopressin (AVP) in cattle

牛注射精氨酸加压素 (AVP) 后应激对血浆 ACTH 和皮质醇浓度的影响

• 批准号: 10660286
• 负责人: HASEGAWA Nobumi
• 金额: $ 2.3万
• 依托单位: MIYAZAKI UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10660286/
• 关键词: Cattl; AVP; ACTH; Cortisol; Social stress; Heat stress; 加齢; ストレス; コルチゾール; 優劣順位

The effect of stress on the plasma concentration of ACTH and cortisol after administration of Arginin-Vasopressin (AVP) on cattle was investigated.Experiment 1. The effect of social stressFive Holstein heifers (Mx) were mixed into the herd of 5 dry cows and 3 heifers in original heifer herd as control (Cn). On 3 days before mixing, 3, 7, 14, 21 days after mixing, blood samples of hefers were collected by venous puncture just before AVP injection, 10, 30, 60 and 120 min. after the injection for plasma ACTH and cortisol measurements. Change of dominance value (ADV) of each heifer was evaluated by win and loss numbers of aggressions between cattle before and after mixing. ADV of MX decreased after mixing. Plasma ACTH concentrations of MX were not different between days. The increase of cortisol concentration after AVP administration of MX became smaller after mixing but that of Cn did not differ among experimental days. ADV had significantly positive correlation with plasma ACTH concentration (p ≦ 0.05).Experiment 2. The effect of heat stress5 heifers (HS) were kept in a outside paddock during 2 hours on a sunny summer day (highest air temperature, 38.0 ℃) and 5(NM) were in barn (34.7 ℃). Blood samples of hefers were collected by venous puncture just before AVP injection, 5, 15, 30, 60 and 120 min. after the injection for plasma ACTH and cortisol measurements. Plasma ACTH concentration did not differ between the treatments. When the two abnormal data were eliminated, ACTH concentration on 5 and I5min tended to be lower in HS than that in NM (p = 0.06). Plasma cortisol concentration did not differ between HS and MN.These results show that blood ACTH and cortisol concentrations of cattle after AVP administration may change under stressful conditions. This method may be useful to evaluate the stress of cattle.

研究了精氨酸蛋白 - 毒素（AVP）对牛的压力对ACTH和皮质醇的血浆浓度的影响。混合前的3天，在混合后3、7、14、21天，在AVP注射之前，通过静脉穿刺收集了甲基血液样本，即10、30、60和120分钟。注射血浆ACTH和皮质醇测量后。通过胜利和混合后牛之间的侵略性损失数量来评估每个小母牛的主导价值变化（ADV）。混合后MX的ADV降低。在几天之间，血浆ACTH浓度MX没有差异。混合后，AVP施用MX后皮质醇浓度的增加变小，但CN的浓度在实验日之间没有差异。 ADV与血浆ACTH浓度具有显着正相关（P≦0.05）。实验2。在阳光明媚的夏日（最高空气温度，38.0℃）和5（nm）中，热应激5小母牛（HS）的影响在2小时内将其保存在外部围场中。在AVP注射之前，通过静脉穿刺收集人的血液样本，即5、15、30、60和120分钟。注射血浆ACTH和皮质醇测量后。血浆ACTH浓度在两种处理之间没有差异。当消除两个异常数据时，在HS中，ACTH的浓度趋于HS中的ACTH浓度低于NM（p = 0.06）。 HS和MN之间的血浆皮质醇浓度没有差异。这些结果表明，在压力条件下，AVP给药后的牛的血液ACTH和皮质醇浓度可能会发生变化。该方法可能有助于评估牛的压力。