The effect of stress on the plasma ACTH and cortisol concentrations after administration of Arginine-Vasopressin (AVP) in cattle

牛注射精氨酸加压素 (AVP) 后应激对血浆 ACTH 和皮质醇浓度的影响

• 批准号: 10660286
• 负责人: HASEGAWA Nobumi
• 金额: $ 2.3万
• 依托单位: MIYAZAKI UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10660286/
• 关键词: Cattl; AVP; ACTH; Cortisol; Social stress; Heat stress; 加齢; ストレス; コルチゾール; 優劣順位

The effect of stress on the plasma concentration of ACTH and cortisol after administration of Arginin-Vasopressin (AVP) on cattle was investigated.Experiment 1. The effect of social stressFive Holstein heifers (Mx) were mixed into the herd of 5 dry cows and 3 heifers in original heifer herd as control (Cn). On 3 days before mixing, 3, 7, 14, 21 days after mixing, blood samples of hefers were collected by venous puncture just before AVP injection, 10, 30, 60 and 120 min. after the injection for plasma ACTH and cortisol measurements. Change of dominance value (ADV) of each heifer was evaluated by win and loss numbers of aggressions between cattle before and after mixing. ADV of MX decreased after mixing. Plasma ACTH concentrations of MX were not different between days. The increase of cortisol concentration after AVP administration of MX became smaller after mixing but that of Cn did not differ among experimental days. ADV had significantly positive correlation with plasma ACTH concentration (p ≦ 0.05).Experiment 2. The effect of heat stress5 heifers (HS) were kept in a outside paddock during 2 hours on a sunny summer day (highest air temperature, 38.0 ℃) and 5(NM) were in barn (34.7 ℃). Blood samples of hefers were collected by venous puncture just before AVP injection, 5, 15, 30, 60 and 120 min. after the injection for plasma ACTH and cortisol measurements. Plasma ACTH concentration did not differ between the treatments. When the two abnormal data were eliminated, ACTH concentration on 5 and I5min tended to be lower in HS than that in NM (p = 0.06). Plasma cortisol concentration did not differ between HS and MN.These results show that blood ACTH and cortisol concentrations of cattle after AVP administration may change under stressful conditions. This method may be useful to evaluate the stress of cattle.

研究了应激对牛精氨酸-抗利尿素（AVP）处理后血浆ACTH和皮质醇浓度的影响。实验1。将5头荷斯坦小母牛（Mx）混入5头干母牛和3头原母牛中作为对照（Cn）。分别于混合前3天、混合后3、7、14、21天，分别于注射AVP前、注射后10、30、60、120分钟静脉穿刺采血，测定血浆ACTH和皮质醇水平。通过混合前后牛间攻击的得失数来评价每头小母牛的优势值变化。混合后MX的ADV减小。MX的血浆ACTH浓度在不同天之间没有差异。AVP给药后MX混合后皮质醇浓度升高幅度减小，Cn给药后各实验天皮质醇浓度升高幅度无显著差异。ADV与血浆ACTH浓度有显著正相关（p≦0.05）。实验2。热应激的影响选择夏季晴天（最高气温38.0℃）5头母牛（HS）在室外围场饲养2 h， 5头母牛（NM）在谷仓饲养（34.7℃）。分别于AVP注射前、注射后5、15、30、60、120 min静脉穿刺取血，测定血浆ACTH和皮质醇水平。两组间血浆ACTH浓度无差异。排除两种异常数据后，HS在5、I5min时ACTH浓度有低于NM的趋势（p = 0.06）。血浆皮质醇浓度在HS和MN之间没有差异。这些结果表明，在应激条件下，AVP给药后牛血ACTH和皮质醇浓度可能发生变化。该方法可用于评价牛的应激状态。