Study of Helicobacter pylori infection using G-CSF transgenic mice.

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1. Colonization ability of various bacteria gnotobiot mice :Colonization of various enterobacteria and Helicobacter pylori in the stomach, intestine and oral cavity was examined using gnotobiot mice, because colonization of Helicobacter pylori has been reported to be inhibited by normal bacterial flora in conventional mice. Eleven bacterial strains were inoculated to germ free mice. These bacteria could colonize in stomach, intestine and oral cavity. However, number of bacteria in each site was various among bacterial strain. For example, large number of Helicobacter pylori could be isolated from stomach while only small number of it could isolated from oral cavity.2. Infection of Helicobacter pylori in transgenic mouse: Transgenic mice were inoculated with Helicobacter pylori Sydney strain 1 (SS1). The strain could colonize in the stomach of these mice. The number of SS1 was decreased at 10 weeks after infection. The inflammation in the stomach was appeared at 2 week after infection/ It is earlier than that in control mice.2. Infection3. Lypopolysaccharide from Helicobacter pylori could induce cytokine synthesis from endothelial cells.