Experimental Study of Metastasis and Invasion Related Oncogene by Using Oral Cancer Cell Lines

据推测，细胞外基质的分解对于癌细胞的转移是必需的，并且基质变蛋白酶的表达是必需的。据报道，ETS结合domein位于这些基因上游的转录调控区，从口腔恶性肿瘤组织中提取蛋白，Western-blot分析E1 AF蛋白的表达。有部分细胞表达E1 AF蛋白，但无强表达细胞。也就是说，有许多癌细胞中E1 AF mRNA强表达，但没有E1 AF蛋白强表达的癌细胞。因此，我们怀疑E1 AF蛋白表达很低或分解的可能性。 ...更多信息 因为在癌细胞中稳定性较低。将逆转录病毒增强子/启动子下的E1 AF基因转染到不表达E1 AF的癌细胞中，并建立生产细胞系。裸鼠皮下注射实验表明，该细胞的转移能力和迁移能力明显增强。这些结果支持了E1 AF基因对恶性肿瘤的侵袭和转移有重要影响的假说，同时对MMP蛋白（MMP 3和MMP 9蛋白）的表达进行了分析。我们研究了这些基因的表达与肿瘤的组织病理学类型（恶性程度）和预后的关系，一般认为E1 AF基因的蛋白表达越强，肿瘤的恶性程度越高（预后越差）。(The MMP蛋白如MM 3和MMP 9蛋白的表达也较高），并且这些基因的表达在许多情况下与癌细胞系的恶性程度和转移能力有很大关系。少

It is assumed that decomposing matrix outside the cell is necessary for the metastasis of the cancer cells, and the expression of the matrix metaroprotease (henceforth MMP) is important for it. It had been reported that the ETS binding domein was located in the transcriptional regulation area in the upstream of these genes, and which played an important role in the transcriptional regulation of these genes by binding of ets-oncogene family products.The protein was extracted from oral malignant tumors (malignant squamous cell carcinoma cell lines), and the expressions of the E1AF protein were studied by the Western-blot analysis. There were some cell lines in which E1AF protein was expressed, but no cell lines of strong protein expression. In other words, there were many cancer cells in which E1AF mRNA was strongly expressed, but no cancer cells of strong protein expression of E1AF. So, we suspected that there was some possibility that the E1AF protein expression was very low or decompo … More sed soon because of that low stability in cancer cells. The E1AF gene under the retrovirus enhancer/promoter was transfected into the cancer cells in which E1AF was not expressed, and the producer cell lines were established. The abilities of metastasis and mobility were elevated in the experiment of injecting these cells to nude mouse subcutaneously. These data support the hypothesis that the metastasis and invasion of a malignant tumor had deeply been affected by E1AF gene.The analysis of the expressions such as the MMP proteins (MMP3 and MMP9 proteins) was also carried at the same time. We investigated the expressions of these genes, the histopathological type of cancer (malignant degree), and the prognosis, and the relation between them was also examined.It was generally thought that the stronger protein expression of E1AF gene was, the more malignant the cancer was (the worse prognosis was). (The expressions of the MMP proteins such as MM3 and MMP9 protein were also high at the same time), and the expressions of these genes have much relation to the malignant degree of cancer and the metastasis ability in many cases of cancer cell lines. Less