Optical study on the generation of experimental arrhythmias with re-entry of the excitatory waves

兴奋波重入导致实验性心律失常产生的光学研究

• 批准号: 10670632
• 负责人: SAKAI Tetsuro
• 金额: $ 2.05万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10670632/
• 关键词: electrical activity; circus movement; optical recording; voltage-sensitive dye; heart; arrhythmia

Multiple-site optical recordings of transmembrane voltage activity, using a voltage-sensitive dye and a multi-element photodiode array, were employed to monitor the tachycardia-like excitation in the rat atrium. Atrial preparations dissected from adult rat hearts were stained with a merocyanine-rhodanine dye (NK2761), and the action potentials were recorded simultaneously from many sites. We used a bathing medium containing 2,3-butanedione monoxime (20 mM) to reduce the contraction-related light scattering. The tachycardia-like excitation with a cycle length of about 250 - 450 ms was evoked either by a train of electrical stimuli (5-20 Hz/ 1-2 sec) or regular stimuli of 0.5-1 Hz applied with bipolar electrodes. The events lasted for various time periods (10 sec - over 10 min) and often terminated spontaneously. The spread of excitation was assessed optically, and we mapped the conduction patterns of the excitatory waves. These maps indicate that the excitatory waves often propagated in a quasi-circular pathway which often surrounded the ostium of the superior vena cava. However, several other patterns, such as "blocked areas" without anatomical obstacle, ectopic foci of pacemaking activity and more complex patterns with multiple wavefront, were also observed.

采用电压敏感染料和多元件光电二极管阵列多点光学记录跨膜电压活动，监测大鼠心房的心动过速样兴奋。从成年大鼠心脏解剖的心房制备物用部花青-若丹宁染料（NK 2761）染色，并从多个部位同时记录动作电位。我们使用含有2，3-丁二酮单肟（20 mM）的沐浴介质，以减少收缩相关的光散射。通过一系列电刺激（5-20 Hz/ 1-2 sec）或用双极电极施加的0.5-1 Hz的常规刺激诱发具有约250 - 450 ms周期长度的心动过速样兴奋。这些事件持续不同的时间段（10秒-超过10分钟），通常自发终止。兴奋的传播进行了光学评估，我们映射的兴奋波的传导模式。这些图表明，兴奋波往往在一个准圆形的途径，往往包围了上级腔静脉口传播。然而，也观察到其他几种模式，如无解剖障碍的“阻塞区”，起搏活动的异位病灶和具有多个波前的更复杂的模式。

项目成果

Obaid A L, Koyano T, Lindstrom J, Sakai T, Salzberg B M.: "Spatiotemporal patterns of activity in an intact mammalian network with single-cell resolution : Optical studies of nicotinic activity in an enteric plexus."J. Neurosci.. 19. 3073-3093 (1999)

Obaid A L、Koyano T、Lindstrom J、Sakai T、Salzberg B M.：“具有单细胞分辨率的完整哺乳动物网络中的活动时空模式：肠丛中烟碱活性的光学研究。”J。

Sato K, Momose-Sato Y, Hirota A, Sakai T, Kamino K.: "Optical mapping of neural responses in the embryonic rat brainstem with reference to the early functional organization of vagal nuclei."J. Neurosci.. 18. 1345-1362 (1998)

Sato K、Momose-Sato Y、Hirota A、Sakai T、Kamino K.：“胚胎大鼠脑干神经反应的光学映射，参考迷走神经核的早期功能组织。”J。

Sakai T.,: "A regional gradient of cardiac intrinsic rhythmicity depicted in embryonic cultured multiple hearts"Pflugers Archiv : European Journal of Physiology. 437. 61-69 (1998)

Sakai T.，：“胚胎培养的多颗心脏中描绘的心脏内在节律性的区域梯度”Pflugers Archive：欧洲生理学杂志。

Obaid A.L.,: "Spatiotemporal Patterns of Activity in an Intact Mammalian Network with Single-Cell Resolution : Optical Studies of Nicotinic Activity in an Enteric Plexus"Journal of Neuroscience. 19. 3073-3093 (1999)

Obaid A.L.，：“具有单细胞分辨率的完整哺乳动物网络中的活动时空模式：肠丛中烟碱活性的光学研究”神经科学杂志。

Sakai,T.: "Optical monitoring of synaptic transmission in bullfrog sympathetic ganglia using a voltage-sensitive dye." Neuroscience Letters. 242. 1-4 (1998)

Sakai,T.：“使用电压敏感染料对牛蛙交感神经节中的突触传递进行光学监测。”