Optical mapping of neuronal activities in the submucous plexus with single cell resolution

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In order to identify functional units of the neural network within the submucous plexus of the guinea pig, multiple-site optical recording of transmembrane voltage activity (MSORTV) with single cell resolution, using a voltage-sensitive styryl dye (di-8-ANEPPS) and a 464 element photodiode array, were employed to monitor the spontaneous electrical activity of the neurons of which the separate ganglia in the plexus are composed. Spike sorting analysis of the raw optical data is used to identify the active neurons and isolate their individual spike trains. Computer generated animations are used to show the patterns of neuronal activity. Preliminary observations of quasicoincident bursts recorded from neurons in separate ganglia suggest the existence of supraganglionic neuronal networks, which form the functional units of the submucous plexus.