Optical mapping of neuronal activities in the submucous plexus with single cell resolution

单细胞分辨率的粘膜下神经丛神经元活动的光学映射

• 批准号: 13670036
• 负责人: SAKAI Tetsuro
• 金额: $ 2.3万
• 依托单位: Tokyo Medical and Dental Universily
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670036/
• 关键词: electrical activity; submucous plexus; optical recording; voltage-sensitive dye; ganglion; styryl

In order to identify functional units of the neural network within the submucous plexus of the guinea pig, multiple-site optical recording of transmembrane voltage activity (MSORTV) with single cell resolution, using a voltage-sensitive styryl dye (di-8-ANEPPS) and a 464 element photodiode array, were employed to monitor the spontaneous electrical activity of the neurons of which the separate ganglia in the plexus are composed. Spike sorting analysis of the raw optical data is used to identify the active neurons and isolate their individual spike trains. Computer generated animations are used to show the patterns of neuronal activity. Preliminary observations of quasicoincident bursts recorded from neurons in separate ganglia suggest the existence of supraganglionic neuronal networks, which form the functional units of the submucous plexus.

为了确定豚鼠粘膜下神经丛神经网络的功能单位，采用电压敏感的苯乙烯染料（di-8-ANEPPS）和464元件光电二极管阵列，采用单细胞分辨率的跨膜电压活动多点光学记录（MSORTV）来监测神经元的自发电活动，神经丛中的分离神经节由这些神经元组成。原始光学数据的尖峰分选分析用于识别活动神经元并分离其单独的尖峰序列。计算机生成的动画用于显示神经元活动的模式。从不同神经节中的神经元记录的准偶发性爆发的初步观察表明存在神经节上神经元网络，其形成粘膜下神经丛的功能单位。

项目成果

Sakai, T.: "In vitro model of atrial arrhythmiai optical mapping of tachycardia-Like excitation in the rat atrium"Japanese Journal of Physiology. 52(Suppl). S73 (2002)

Sakai，T.：“房性心律失常的体外模型，大鼠心房中心动过速样激励的光学映射”，日本生理学杂志。

Sakai T.: "Optical mapping of excitation spread during tachycardia-like excitation in the isolated rat atrium"American Journal of Physiology. submitted.

Sakai T.：“离体大鼠心房中心动过速样激发过程中激发传播的光学映射”美国生理学杂志。

Sakai T., Kamino K.: "Optical mapping approaches to cardiac electrophysiological functions"Japanese Journal of Physiology. 51. 1-18 (2001)

Sakai T.，Kamino K.：“心脏电生理功能的光学映射方法”日本生理学杂志。

Sakai T.: "Optical imaging of the spatiotemporal patterns of the excitation spread in the atrium using a voltage-sensitive dye"Japanese Journal of Physiology. 51 (suppl.). S49 (2001)

Sakai T.：“使用电压敏感染料对心房中激发传播的时空模式进行光学成像”日本生理学杂志。

Tetsuro Sakai: "Optical imaging of the spatioemporal patterns of the excitation spread in the atrium using a voltage-sensitive clye"Japanese Journal of Physiology. 51(suppl.). 549 (2001)

Tetsuro Sakai：“使用电压敏感 clye 对心房中兴奋传播的时空模式进行光学成像”《日本生理学杂志》。