Optical imaging analysis of the functional organization of the receptor-intracellular signal transduction system in the early embryonic heart.

早期胚胎心脏受体细胞内信号转导系统功能组织的光学成像分析。

• 批准号: 16590166
• 负责人: SAKAI Tetsuro
• 金额: $ 2.24万
• 依托单位: University of the Ryukyus
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16590166/
• 关键词: Optical recording; Voltage-sensitive dye; Automatism; Receptor; Intracellular signal transduction; Functional organization; Imaging

Voltage-sensitive dye-related absorption signals have provided a new method for monitoring spontaneous electrical activity in the early embryonic heart. We have introduced multiple-site optical recording of transmembrane voltage activity, using a fast merocyanine-rhodanine voltage-sensitive dye (NK2761) and a multi-element (16 X 16) photodiode array, to monitor action potentials in the early embryonic chick hearts at the stages when the first muscle contractions appear. By the application of adrenaline, the rhythm of the cardiac pacemaker became faster although the effect of this drug was very weak in comparison with that in adult hearts. On the other hand, the application of caffeine accelerated the rhythm of the pacemaker, suggesting that the intracellular signal transduction system was partially functioning. However, the application of adrenaline slowed the conduction velocity of the excitatory wave from the pacemaker. These results suggest that, in the early embryonic heart, the intracellular signal transduction system is somewhat different from that of adult hearts.

电压敏感染料相关吸收信号为监测早期胚胎心脏自发电活动提供了一种新的方法。我们采用快速部花青-若丹宁电压敏感染料（NK 2761）和多元件（16 × 16）光电二极管阵列，在鸡胚心脏第一次肌肉收缩出现的阶段，采用多位点光学记录跨膜电压活动，监测鸡胚心脏的动作电位。应用肾上腺素后，心脏起搏器的节律变快，尽管这种药物的作用与成人心脏相比很弱。另一方面，咖啡因的应用加快了起搏器的节律，表明细胞内信号转导系统部分发挥作用。然而，肾上腺素的应用减慢了起搏器兴奋波的传导速度。这些结果表明，在早期胚胎心脏，细胞内的信号转导系统是有些不同的成人心脏。

项目成果

Action Spectra of Voltage -sensitive Dyes in the Isolated Rat Atrium

电压敏感染料在离体大鼠心房中的作用谱

• 发表时间: 2005
• 期刊: Japanese Journal of Physiology 55・Suppl.
• 作者: SAKAI;T
• 通讯作者: T

Use of cytochalasin D as the uncoupler of EC coupling for the optica monitoring of action potentials in the isolatcd rat atrium

使用细胞松弛素 D 作为 EC 偶联解偶联剂用于离体大鼠心房动作电位的光学监测

• 发表时间: 2006
• 期刊: Journal of Physiological Sciences 56・Suppl
• 作者: SAKAI;T
• 通讯作者: T

Optical Mapping of Tachycardia-like Excitation Evoked by Pacing in the Isolated Rat Atrium

离体大鼠心房起搏引起的心动过速样兴奋的光学测绘

• 发表时间: 2005
• 期刊: Japanese Journal of Physiology 54・6
• 作者: SAKAI;T
• 通讯作者: T

Use of Cytochalasin D as the Uncoupler of E-C Coupling for the Optical Monitoring of Action Potentials in the Isolated Rat Atrium.

使用细胞松弛素 D 作为 E-C 耦合的解偶联剂，用于离体大鼠心房动作电位的光学监测。

• 发表时间: 2006
• 期刊: Journal of Physiological Sciences 56・Suppl
• 作者: SAKAI;T
• 通讯作者: T

A regional gradient of intrinsic rhythmicity depicted in embryoni cultured muliple-hearts.

胚胎培养的多心脏中描绘的内在节律性的区域梯度。

• 发表时间: 2006
• 期刊: Seibutsu Butsuri 46・Supp12
• 作者: SAKAI;T
• 通讯作者: T