Role of Chemokines in Acute Rejection after Rat Lung Transplantation. Interaction with Nitric Oxide and Endothelin.

趋化因子在大鼠肺移植后急性排斥反应中的作用。

• 批准号: 10671257
• 负责人: MINAMI Masato
• 金额: $ 1.98万
• 依托单位: Osaka University Graduate School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671257/
• 关键词: Rat lung transplantation; Nitric Oxide; iNOS; Acute Rejection; BAL cell; 急性拒絶反応; ラット; 肺移植; 拒絶反応; ケモカイン; NO; エンドセリン

Rat lung transplantation was performed in isografts and allografts. In isografts, no acute rejection and no significant increase in NO production was identified. In allografts, grade I-II of acute rejection was seen on postoperative day (POD) 3 and grade III on POD 5. NO produced by BAL cells increased on both POD 3 (11.8±2.0 parts per billion [ppb] and POD 5 (115.3±66.9 ppb). There was a highly significant correlation between the level of NO and the severity of AR (p<0.005). BAL cells from allografts expressed iNOS mRNA.Among them, macrophages, lymphocytes and neutrophils were immunostained for iNOS.In conclusion, NO production by BAL cells was detected in the early stages of rejection. Therefore it may serve as a sensitive indicator of AR in lung transplantation.

大鼠肺移植分为同系和同种异体两种。在同种异体移植物中，没有发现急性排斥反应，也没有发现NO产生的显著增加。在同种异体移植物中，术后第3天（POD）观察到I-II级急性排斥反应，术后第5天观察到III级。BAL细胞产生的NO在POD 3（11.8±2.0 ppb）和POD 5（115.3±66.9 ppb）均增加。NO水平与AR的严重程度呈显著正相关（p<0.005）。免疫组化结果显示，同种异体移植物的BAL细胞表达iNOS mRNA，巨噬细胞、淋巴细胞和中性粒细胞表达iNOS。因此，它可以作为肺移植AR的敏感指标。

项目成果

T Utsumi: "Nitric oxide production by bronchoalveolar cells during allograft rejection in the rat"Transplantation. 67・12. 1622-1626 (1999)

T Utsumi：“大鼠同种异体移植排斥过程中支气管肺泡细胞产生的一氧化氮”67・12 1622-1626（1999）。

T Utsumi. M Minami.: "Nitric oxide production by bronchoalveolar cells during allograft refection in the rat"Transplantation. 67. 1622-1626 (1999)

内海 T.