Diagnosis and monitoring of acute rejection after lung transplantation

肺移植术后急性排斥反应的诊断和监测

• 批准号: 17591469
• 负责人: MINAMI Masato
• 金额: $ 1.98万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591469/
• 关键词: lung transplantation; rejection; peripheral blood lymphocyte; pneumonia; activation marker

Accurate diagnosis of acute rejection, reperfusion injury, or pulmonary infection is difficult in the postoperative acute phase in the lung transplantation. We investigate the clinical utility of flow cytometry for surface antigen (CD3, CD4, CD8, CD25, CD69, CD45RA, CD45RO) on peripheral blood lymphocytes in order to diagnose the posttransplant status of patients. Also, the effect of anti-CD25 antibody administration was discussed. We found no evident change of CD4/CD8 ratio, CD25 expression, CD69 expression, and the proportion of CD45RA/RO in the patient with stable course. CD28 expression level decreased during the 1st month after transplant and this tendency was predominantly found in CD8 T cells as compared to CD4 subset. In a patient suffering interstitial pneumonia, increase of CD25 expression in CD4 T cells and increase of CD69 expression in CD8 T cells were shown. CD25 expression might be suppressed by CD25 antibody administration.

在肺移植术后急性期，准确诊断急性排斥反应、再灌注损伤或肺部感染是困难的。我们研究了流式细胞术检测外周血淋巴细胞表面抗原（CD 3、CD 4、CD 8、CD 25、CD 69、CD 45 RA、CD 45 RO）的临床应用，以诊断移植后患者的状态。此外，抗CD 25抗体的管理的影响进行了讨论。病程稳定者CD_4/CD_8比值、CD_（25）表达、CD_（69）表达及CD_（45）RA/RO比值无明显变化。CD 28表达水平在移植后1个月内下降，与CD 4亚群相比，这种趋势主要见于CD 8 T细胞。在患有间质性肺炎的患者中，显示出⑶ 4 T细胞中的⑶ 25表达增加和⑶ 8 T细胞中的⑶ 69表达增加。CD 25表达可能受到CD 25抗体给药的抑制。