Analysis of Angiogenesis for the Intra-Articular Lesions of Temporomandibular Joint Disorders by Mechanical Stress

机械应力引起的颞下颌关节疾病关节内病变的血管生成分析

• 批准号: 10671773
• 负责人: SATO Tsuyoshi
• 金额: $ 1.92万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671773/
• 关键词: Temporomandibular Joint Disorders; Joint Pain; Posterior Disk Attachment; MR Images; Angiogenesis; Estrogen; Cytokine; Nitric Oxide; TMJ

Analysis of Intra-Articular Inflammatory changes for Temporomandibular Joint DisordersThe present study evaluated the relationship between joint pain, contrast enhancement of the posterior disk attachment by MR imaging, and nitric oxide production in the synovial fluid of patients with TMJ disorders.Subjective pain level and location were determined by the patient's self assessment using the 100 mm visual analog scale. Synovial fluid was collected from the superior compartment of the joint using a direct aspiration technique. The level of nitric oxide production in the synovial fluid was measured by the Griess reaction.The degree of pain was correlated with contrast enhancement of the posterior disk attachment (n=40, r=0.70, P<0.001). The elevated level of nitric oxide in synovial fluid was associated with high degree of joint pain and prominent enhancement of the posterior disk attachment. Nitric oxide may be a critical mediator that functions to clarify the inflammatory cascade in the TMJ region.Effects or Estrogen on cytokine Secretion and Nitric Oxide production from Human Synovial FibroblastsIn the present study, we investigated the molecularbiological effects of estrogen on inflammatory cytokine production, nitric oxide production, and iNOS mRNA expression using the synovial tissues of femoral joint from a patient with rheumatoid arthritis.17b-estradiol significantly inhibited GM-CSF secretion and nitrite production in the culture medium in a concentration-dependent manner which reached significance at 10 mM (p<0.05). Using RT-PCR techniques the expression of the iNOS mRNA could be demonstrated in unstimulated and cytokine-stimulated cultures of synovial fibroblasts in the presence of estradiol (10 mM). Our results suggest that synovial fibroblasts are sensitive to the influence of estrogen and provide information for their role as immunomodulatory factors. Estrogen indeed might exert protective effects in the inflamed joint.

颞下颌关节紊乱病关节内炎性改变的分析本研究评估了关节疼痛、MR成像后盘附着增强和TMJ紊乱病患者滑液中一氧化氮产生之间的关系，患者使用100 mm视觉模拟量表进行自我评估，确定主观疼痛程度和部位。使用直接抽吸技术从关节的上级室收集滑液。采用Griess反应法测定关节液中一氧化氮的含量，疼痛程度与椎间盘后附着区的增强程度相关（n=40，r=0.70，P<0.001）。关节液中一氧化氮水平的升高与关节疼痛程度的增加和椎间盘后部附着的显著增强有关。本研究探讨了雌激素对人滑膜成纤维细胞分泌炎性细胞因子、一氧化氮及一氧化氮合酶的分子生物学效应，并探讨了雌激素对人滑膜成纤维细胞分泌炎性细胞因子、一氧化氮及一氧化氮合酶的影响。和iNOSmRNA表达。17 β-雌二醇显著抑制GM-CSF分泌和培养液中亚硝酸盐的产生，其浓度为依赖性方式，在10 mM时达到显著性（p<0.05）。使用RT-PCR技术的iNOS mRNA的表达，可以证明在未刺激和姜黄素刺激的滑膜成纤维细胞的培养物中的雌二醇（10 mM）的存在下。我们的研究结果表明，滑膜成纤维细胞的雌激素的影响是敏感的，并提供信息，其作用作为免疫调节因子。雌激素确实可能在发炎的关节中发挥保护作用。