Does semaphorin regulate differentiation and network among cells in osteocyte?

我们发现骨细胞表达丛蛋白A1、A2、A3、B1、B2、C1，丛蛋白A1和丛蛋白A2 mRNA的表达均依次增加。骨细胞产生信号蛋白3A。信号蛋白3A诱导骨细胞中E11、连接蛋白43和DMP1的表达。通过软x线分析，我们检测到具有信号蛋白3A的水凝胶作用的小鼠股骨与对照组相比骨矿物质含量较高。综上所述，我们发现信号蛋白3A在体外调节骨细胞的分化和树突延伸，并促进骨在体内的成熟。

We have found that osteocytes express plexin A1, A2, A3, B1, B2, C1 and both plexin A1 and plexin A2 increase mRNA expression sequentially. Osteocytes produce semaphorin 3A protein. Semaphorin 3A induces the expression of E11, connexin 43 and DMP1 in osteocyte. Using soft x-ray analysis, we detected high bone mineral in mice whose femur effected with hydrogel with semaphorin 3A in comparison with the control. Taken together, we discovered that semaphorin 3A regulates differentiation and dendrite extension on osteocytes in vitro and also promotes maturation of bone in vivo.