Does semaphorin regulate differentiation and network among cells in osteocyte?

我们发现骨细胞表达丛蛋白A1、A2、A3、B1、B2、C1，并且丛蛋白A1和丛蛋白A2的mRNA表达依次增加。骨细胞产生semaphorin 3A蛋白。Semaphorin 3A诱导骨细胞表达E11、Cx43和DMP1使用软X射线分析，我们检测到高骨矿物质在小鼠的股骨与水凝胶与semaphorin 3A的影响，与对照组相比。综上所述，我们发现semaphorin 3A在体外调节骨细胞的分化和树突延伸，并在体内促进骨成熟。

We have found that osteocytes express plexin A1, A2, A3, B1, B2, C1 and both plexin A1 and plexin A2 increase mRNA expression sequentially. Osteocytes produce semaphorin 3A protein. Semaphorin 3A induces the expression of E11, connexin 43 and DMP1 in osteocyte. Using soft x-ray analysis, we detected high bone mineral in mice whose femur effected with hydrogel with semaphorin 3A in comparison with the control. Taken together, we discovered that semaphorin 3A regulates differentiation and dendrite extension on osteocytes in vitro and also promotes maturation of bone in vivo.