Analysis and Biomedical Material Applications of Biological Hard Tissue Formation Based on Electromagnetics

羟基磷灰石是一种天然的生物活性物质，因此骨骼是由其表面的成骨细胞生成的，没有任何包裹体。实验证明，植入后1周即可形成骨，伤口可在3周内修复。然而，由于羟基磷灰石与骨之间有很大的间隙，羟基磷灰石被纤维组织包裹，没有与骨结合。我们最近的研究表明，电场极化在羟基磷灰石表面产生的负电荷通过模拟体液浸泡的方式加速了类骨磷灰石的过度生长。在这里，我们显示了带负电荷的表面的电极化羟基磷灰石陶瓷促进了犬骨髓的成骨。植入后7天，带负电荷的羟基磷灰石表面在大间隙环境中进行骨形成。到第14天，约0.2毫米的间隙被与羟基磷灰石表面刚性接触的成熟骨填充。静电力对髓系细胞骨形成的激活和负电荷表面c面高取向羟基磷灰石层的形成都有影响。

Hydroxyapatite is an innate bioactive material such that bones are generated by osteoblasts on its surface without any inclusion. It has been experimentally established that bone formation occurs 1 week after implantation, and the wound is repaired within 3 weeks. However, provided a wide gap between the hydroxyapatite and bone, the hydroxyapatite is encapsulated with fibrous tissues and not integrated with the bones. Our recent studies demonstrated that the negative electrical charges on the hydroxyapatite surfaces induced by electrical polarization accelerated the bone-like apatite overgrowth by means of simulated body fluid immersion. Here we show that the negatively charged surfaces of the electrically polarized hydroxyapatite ceramics enhanced the osteogenesis of canine bone marrow. The negatively charged hydroxyapatite surface conducted bone formation 7 days after implantation in the widely gapped environment. By day 14, a ca. 0.2 mm gap was filled with maturing bone rigidly contacted with the hydroxyapatite surfaces. The electrostatic force was revealed to have effects both upon activation of the bone formation by myeloid cells and on construction on the c-face highly-orientated hydroxyapatite layer on the negatively charged surface.