Analysis and Biomedical Material Applications of Biological Hard Tissue Formation Based on Electromagnetics

羟基磷灰石是一种天然的生物活性材料，因此骨骼是由成骨细胞在其表面生成的，没有任何内含物。经实验证实，植入后1周即可形成骨，3周内伤口即可修复。然而，如果羟基磷灰石和骨之间存在较大的间隙，则羟基磷灰石被纤维组织包裹而不与骨结合。我们最近的研究表明，通过模拟体液浸泡，电极化引起的羟基磷灰石表面的负电荷加速了骨状磷灰石的过度生长。在这里，我们发现电极化羟基磷灰石陶瓷的带负电表面增强了犬骨髓的成骨作用。带负电荷的羟基磷灰石表面在植入宽间隙环境中7天后进行骨形成。到第 14 天，大约。 0.2毫米的间隙被与羟基磷灰石表面刚性接触的成熟骨填充。研究表明，静电力对骨髓细胞骨形成的激活以及带负电表面上的 c 面高度定向羟基磷灰石层的构建都有影响。

Hydroxyapatite is an innate bioactive material such that bones are generated by osteoblasts on its surface without any inclusion. It has been experimentally established that bone formation occurs 1 week after implantation, and the wound is repaired within 3 weeks. However, provided a wide gap between the hydroxyapatite and bone, the hydroxyapatite is encapsulated with fibrous tissues and not integrated with the bones. Our recent studies demonstrated that the negative electrical charges on the hydroxyapatite surfaces induced by electrical polarization accelerated the bone-like apatite overgrowth by means of simulated body fluid immersion. Here we show that the negatively charged surfaces of the electrically polarized hydroxyapatite ceramics enhanced the osteogenesis of canine bone marrow. The negatively charged hydroxyapatite surface conducted bone formation 7 days after implantation in the widely gapped environment. By day 14, a ca. 0.2 mm gap was filled with maturing bone rigidly contacted with the hydroxyapatite surfaces. The electrostatic force was revealed to have effects both upon activation of the bone formation by myeloid cells and on construction on the c-face highly-orientated hydroxyapatite layer on the negatively charged surface.