Epidemiological study and candidate gene analysis of cleft lip and /or plate in Japanese patients

1.北里大学25年来467例唇裂和/或唇板裂患者的流行病学研究（1）性别，（2）手术年龄，（3）出生地，（4）多因素疾病，（5）出生顺序，（6）家系出现率和兄弟出现率，（7）裂的分类（双侧/单侧唇腭裂、硬软腭裂、软腭裂、粘膜下腭裂）;（8）手术例数的年变化。结果：（1）性别-男：女=249：217（2）手术时年龄-平均：（4）多因素疾病92例，占19.7%;（5）出生顺序：第一胎191例，占40.9%，第二胎203例，占43.5%，第三胎67例，占14.3%，第四胎203例，占40.9%，第四胎203例，占43.5%，第五胎203例，占43.3%，第六胎5例1.1%，（6）家系出现率-16.5%，（7）双侧/单侧唇腭裂：254例43.5%，硬软腭裂：73例15.6%，软腭裂：93例19.9%，粘膜下腭裂：10例10.1%，（8）手术例数年平均变化：20例。各裂位多因素疾病出现率及家系出现率调查：（1）多因素疾病总出现率94/467 20.1%，其中唇腭裂29/251 11.6%，硬软腭裂28/55 50.9%，软腭裂24/92 26.1%，粘膜下腭裂9/48 18.8%。(2)家系出现率-36/218 16.5%。孤立性腭裂多因素综合征的发生率较高（61/195，31.1%）.日本患者唇裂和/或板的候选基因分析：我们正在使用高分辨率DNA分型方法检查日本CL/P患者的HLA I类（HLA-B）和II类等位基因（HLA-DRB 1和DPB 1）。目前，结果还没有出来。

1. Epidemiological study of 467 cleft lip and /or plate patients in Kitasato Univ. 25 years In the past: (1) Sex, (2) Age when operating, (3) Birthplace, (4) Multifactorial diseases, (5) Birth Order, (6) Lineage appearance rate & Brothers appearance rate, (7) Classification of Cleft (bilateral/unilateral cleft lip and palate, cleft hard and soft palate, cleft soft palate, submucous cleft palate), (8) Change at annuals of operation number.Results; (1) Sex- male: female=249:217 (2) Age when operating- average: seven months a years old, (3) Birthplace- Kanagawa Pref, 78.4%, (4) Multifactorial diseases- 92cases 19.7%, (5) Birth Order- the first child: 191cases 40.9%, the second child: 203cases 43.5%, the third child: 67cases 14.3%, the fourth child: 5cases 1.1%, (6) Lineage appearance rate -16.5%, (7) bilateral/unilateral cleft lip and palate: 254cases 43.5%,, cleft hard and soft palate: 73cases 15.6%, cleft soft palate: 93cases 19.9%, submucous cleft palate: 10cases 10.1%, (8) Change at annuals of operation number-average during year: 20cases.2. Investigation of multifactorial diseases appearance rate in each cleft and lineage appearance rate: (1) Multifactorial diseases-Total appearance rate: 94/467 20.1%, cleft lip and palate: 29/251 11.6%, cleft hard and soft palate: 28/55 50.9%, cleft soft palate: 24/92 26.1%, submucous cleft palate: 9/48 18.8%. (2) Lineage appearance rate-36/218 16.5%. High multifactorial diseases appearance rate (61/195 31.1%) was observed in isolated cleft palate.3. Candidate gene analysis of cleft lip and /or plate in Japanese patients:We are examining HLA class I (HLA-B) and class II alleles (HLA-DRB1 and DPB1) in Japanese patients with CL/P using high resolution DNA typing methods. At present, the result has not come out yet.