Epidemiological study and candidate gene analysis of cleft lip and /or plate in Japanese patients

1. 25年来北中大学467例唇腭裂患者的流行病学调查：(1)性别，(2)手术年龄，(3)出生地，(4)多因素疾病，(5)出生顺序，(6)世系外观率及兄弟外观率，(7)唇裂分类（双侧/单侧唇腭裂、软硬腭裂、软腭裂、粘膜下腭裂），(8)手术次数年变化。(1)性别-男：女=249:217(2)手术年龄-平均：7个月，(3)出生地-神奈川县78.4%,(4)多因素疾病- 92例19.7%,(5)出生顺序-第一胎191例40.9%，第二胎203例43.5%，第三胎67例14.3%，第四胎5例1.1%,(6)血统外观率-16.5%,(7)双侧/单侧唇腭裂254例43.5%，软硬裂73例15.6%，软腭裂：1 .黏膜下腭裂93例（19.9%），10例（10.1%）；各腭裂多因素疾病表现率及谱系表现率调查：(1)多因素疾病-总表现率94/467 20.1%，唇腭裂29/251 11.6%，软硬腭裂28/55 50.9%，软腭裂24/92 26.1%，粘膜下腭裂9/48 18.8%。(2)血统出现率-36/218 16.5%。2 .孤立性腭裂多因素疾病发生率高（61/195,31.1%）。日本唇裂和/或平板患者的候选基因分析：我们正在使用高分辨率DNA分型方法检测日本CL/P患者的HLA I类（HLA- b）和II类等位基因（HLA- drb1和DPB1）。目前，结果还没有出来。

1. Epidemiological study of 467 cleft lip and /or plate patients in Kitasato Univ. 25 years In the past: (1) Sex, (2) Age when operating, (3) Birthplace, (4) Multifactorial diseases, (5) Birth Order, (6) Lineage appearance rate & Brothers appearance rate, (7) Classification of Cleft (bilateral/unilateral cleft lip and palate, cleft hard and soft palate, cleft soft palate, submucous cleft palate), (8) Change at annuals of operation number.Results; (1) Sex- male: female=249:217 (2) Age when operating- average: seven months a years old, (3) Birthplace- Kanagawa Pref, 78.4%, (4) Multifactorial diseases- 92cases 19.7%, (5) Birth Order- the first child: 191cases 40.9%, the second child: 203cases 43.5%, the third child: 67cases 14.3%, the fourth child: 5cases 1.1%, (6) Lineage appearance rate -16.5%, (7) bilateral/unilateral cleft lip and palate: 254cases 43.5%,, cleft hard and soft palate: 73cases 15.6%, cleft soft palate: 93cases 19.9%, submucous cleft palate: 10cases 10.1%, (8) Change at annuals of operation number-average during year: 20cases.2. Investigation of multifactorial diseases appearance rate in each cleft and lineage appearance rate: (1) Multifactorial diseases-Total appearance rate: 94/467 20.1%, cleft lip and palate: 29/251 11.6%, cleft hard and soft palate: 28/55 50.9%, cleft soft palate: 24/92 26.1%, submucous cleft palate: 9/48 18.8%. (2) Lineage appearance rate-36/218 16.5%. High multifactorial diseases appearance rate (61/195 31.1%) was observed in isolated cleft palate.3. Candidate gene analysis of cleft lip and /or plate in Japanese patients:We are examining HLA class I (HLA-B) and class II alleles (HLA-DRB1 and DPB1) in Japanese patients with CL/P using high resolution DNA typing methods. At present, the result has not come out yet.