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The Development of Bone Substitute Added with Bone Morphogenetic Protein and Clinical Application

添加骨形态发生蛋白的骨替代品的研制及临床应用

基本信息

批准号：
01870086
负责人：
YAMAZAKI Yasuharu
金额：
$ 1.92万
依托单位：
Yamanashi Medical College
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research (B).
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1990
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01870086/
关键词：
Bone morphogenetic protein Bone induction Bone substitute Plaster of paris Delivery system Bone conduction TGF-β 骨補填材

项目摘要

[Bone Morphogenetic Protein (BMP)]Amino acid sequences of six bone morphogenetic proteins (BMPs) were reported by Wozney, j. et al. The amino acid sequences for our final highly purified protein from bovine bone show considerable homology to BMP-3.[Bone substitute added with BMP]In TCP (Tricalcium phosphate) or HA (Hydroxyapatite) mixed with BMP implanted into the mouse femoral muscle, it was confirmed that BMP induced bone formation around and on the surface of those particles. There were no difference between the yield of new bone of BMP/HA (Hydroxyapatite) or BMP/TCP (Tricalcium phosphate) composite. However, We found the yield of new bone of it was less than that of BMP alone. This would appeared to indicate that BMP was spread within a short period in vivo, and tissue differentiation was disturbed by HA or TCP. Therefore, an effective BMP delivery system from synthetic vehicle for concentrating BMP was required. Then we investigated the morphologic sequence of a response to a BMP/ … More PLP (Plaster of Paris) composite implanted into the mouse femoral muscle. Minimum crude BMP alone dose for bone induction was needed 3mg, on the other hand, in BMP/PLP (1 : 5) composite, that was reduced up to one mg. In more purified BMP (the activity was four times greater than that of crude-BMP), BMP dose was, furthermore, decreased up to 0.25mg in BMP/PLP (1 : 10) composite. Also the BMP/PLP composite implanted into the bone area of rat and monkey induced active bone formation. These results suggest that PLP composite system is one of the suitable BMP delivery systems currently available.[Clinical application]For the observation of clinical usefulness of BMP, autodecalcified dentin, it was already reported that BMP was included in the dentin, was prepared. That powder was implanted into bone defects after resection of oral and maxillofacial diseases. In results, autodecalcified dentin did not cause a foreign body reaction and inflammation, but we did not, contrary to expectation, confirmed bone formation induced by that dentin in roentgenograms. The some cause of it is to be sought that in partly clinical detection methods of induced bone by BMP, almost the deficiency of BMP in autodecalcified dentin and target cell in host tissue. These important problems must be solved in future. Less

[Bone形态发生蛋白（BMP）] Wozney，j.等报道了六种骨形态发生蛋白（BMP）的氨基酸序列。我们最终从牛骨高度纯化的蛋白质的氨基酸序列显示出与BMP-3相当的同源性。[Bone添加有BMP的替代物]在植入到小鼠股肌中的与BMP混合的TCP（磷酸三钙）或HA（羟基磷灰石）中，证实了BMP诱导了这些颗粒周围和表面上的骨形成。BMP/HA（羟基磷灰石）和BMP/TCP（磷酸三钙）复合材料的成骨量无明显差异。但是，我们发现它的新骨产量低于单独的BMP。这似乎表明BMP在体内短时间内扩散，并且组织分化被HA或TCP干扰。因此，需要一种有效的来自合成载体的BMP递送系统来浓缩BMP。然后，我们研究了对BMP/BMP复合物的反应的形态学序列。 ...更多信息将PLP（熟巴黎）复合材料植入小鼠股肌内。BMP/PLP（1：5）复合材料诱导成骨的最小剂量为1 mg。在更纯化的BMP中（活性比粗制BMP大4倍），BMP/PLP（1：10）复合物中BMP剂量进一步降低至0.25mg。BMP/PLP复合材料植入大鼠和猴的骨区也能诱导活性骨形成。这些结果表明，PLP复合系统是目前可用的合适的BMP递送系统之一。[临床应用]为了观察BMP的临床应用价值，制备了BMP-自体脱钙牙本质。将该粉末植入口腔颌面部疾病切除后的骨缺损中。结果，自体脱钙的牙本质没有引起异物反应和炎症，但与预期相反，我们没有在X光片中证实牙本质诱导的骨形成。部分临床应用BMP诱导成骨的检测方法中，几乎都是由于自体脱钙牙本质和宿主组织中的靶细胞缺乏BMP。这些重要问题必须在未来解决。少