The cryopreservation of umbilical cord blood derived mesenchymal stem cells and the possibility of clinical application of those cells to cleft lip and palate patient.

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This research was done with the umbilical cord derived mesenchymal stem cells, because the cord blood derived mesenchymal stem cell couldn't be confirmed. Three months of cryopreservation later, the proliferation potency and multipotency of the umbilical cord derived mesenchymal stem cell were apparently inferior to those of bone marrow derived mesenchymal stem cell. The bone marrow derived mesenchymal stem cell differentiated easily in the osteoblast-like cell under the bone induction medium, but the biological bone viability of the umbilical cord derived mesenchymal stem cell was low, even if bone induction was done with added BMP-2 after the umbilical cord derived mesenchymal cell proliferated with b-FGF. Therefore, as cell source for the regenerated bone, We thought that clinical application of the cryopreserved mesenchymal stem cell from an umbilical cord or blood origin was difficult.