The cryopreservation of umbilical cord blood derived mesenchymal stem cells and the possibility of clinical application of those cells to cleft lip and palate patient.

脐带血间充质干细胞的冷冻保存及其临床应用于唇腭裂患者的可能性。

• 批准号: 20592345
• 负责人: YAMAZAKI Yasuharu
• 金额: $ 2.91万
• 依托单位: Kitasato University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20592345/
• 关键词: 再生医工学; 未分化間葉系幹細胞; 膀帯血; 贋帯; 臍帯血; 臍帯

This research was done with the umbilical cord derived mesenchymal stem cells, because the cord blood derived mesenchymal stem cell couldn't be confirmed. Three months of cryopreservation later, the proliferation potency and multipotency of the umbilical cord derived mesenchymal stem cell were apparently inferior to those of bone marrow derived mesenchymal stem cell. The bone marrow derived mesenchymal stem cell differentiated easily in the osteoblast-like cell under the bone induction medium, but the biological bone viability of the umbilical cord derived mesenchymal stem cell was low, even if bone induction was done with added BMP-2 after the umbilical cord derived mesenchymal cell proliferated with b-FGF. Therefore, as cell source for the regenerated bone, We thought that clinical application of the cryopreserved mesenchymal stem cell from an umbilical cord or blood origin was difficult.

由于脐带血间充质干细胞不能被证实，所以本研究是用脐带间充质干细胞进行的。低温保存3个月后，脐带间充质干细胞的增殖能力和多能性明显低于骨髓间充质干细胞。骨髓间充质干细胞在成骨细胞样细胞中易于分化，但脐带间充质干细胞在b-FGF增殖后加入BMP-2诱导成骨，其生物成骨活力较低。因此，作为再生骨的细胞来源，我们认为从脐带或血液来源冷冻保存的间充质干细胞的临床应用是困难的。

项目成果

Eiju Uchinuma Potential of regenerative medicine of bone tissue using the autologous umbilical cord and umbilical cord blood

Eiju Uchinuma 使用自体脐带和脐带血进行骨组织再生医学的潜力

• 发表时间: 2010
• 期刊: 20th Congress of the European Association of Cranio-Maxillp-Facial Surgery
• 作者: Kyoko Baba;Yasuharu Yamazaki;Kazuya Aoyagi;Akira Takeda
• 通讯作者: Akira Takeda

Proliferative and differential potentials of human bone marrow-derived mesenchymal cells cultured with autologous serum.

自体血清培养的人骨髓间充质细胞的增殖和分化潜能。

• 发表时间: 2010
• 期刊: 20^<th> Congress of the European Asossiation for Cranio-Maxiilo-Facial Surgery(Proceedings)
• 作者: Yamazaki Y.;et al.
• 通讯作者: et al.

proliferative and differential potentials of human bone marrow-derived mescenchymal cells cultured with autologous serum

自体血清培养人骨髓间充质细胞的增殖和分化潜能

• 发表时间: 2010
• 期刊: 20th Congress of the European Association of Cranio-Maxillp-Facial Surger
• 作者: Yasuharu Yamazaki;Akira Takeda;Kyoko Baba;Eiju Uchinuma
• 通讯作者: Eiju Uchinuma

Differing effects of allogenic, heterologous sera and scaffold structures on the bone forming ability of undifferentiated mesenchymal stem cells

同种异体、异源血清和支架结构对未分化间充质干细胞骨形成能力的不同影响

• 发表时间: 2010
• 期刊: The Kitasato Medical Journal 40(2)
• 作者: Chikara Takase;Yasuharu Yamazaki;Aoi Matsuo;Kazuya Aoyagi;Akira Takeda;Eiju Uchinuma
• 通讯作者: Eiju Uchinuma

唇顎裂症例に対する早期顎裂部骨移植の評価

早期唇裂植骨治疗唇颌裂病例的评价

• 发表时间: 2010
• 期刊: 日本口腔外科学会雑誌 56(5)
• 作者: Yasuharu Yamazaki;Akira Takeda;Kyoko Baba;Eiju Uchinuma;山崎安晴,中久木康一,石渡靖夫,瀬崎晃一郎,内沼栄樹
• 通讯作者: 山崎安晴,中久木康一,石渡靖夫,瀬崎晃一郎,内沼栄樹