The reaction mechanism of the ribonuclease T2 family

核糖核酸酶T2家族的反应机制

• 批准号: 10672025
• 负责人: NAKAMURA Kazuo
• 金额: $ 2.11万
• 依托单位: Showa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10672025/
• 关键词: ribonuclease; X-ray structure determination; substrate analogue; subsite; リボ核酸分解酵素

The RNase T2 family shows broad base specificity. The objects of the project on the RNase T2 family are to gain the base-recognition and catalytic mechanism of the RNase T2 family enzymes. The substrate-fragment complexs of RNase LE reveal interesting features : the substrate fragments (3'-GMP and 5'-GMP) and substrate analogue d(GpG) bound in the productive binding mode while inhibitor (2'-GMP) bound in the non-productive binding mode ; and the binding mode of 3'-GMP at the Bi subsite and that of 5'-GMP at the B2 site are similar to that of d(GpG) bound at both B 1 and B2 subsite. These observations show that the binding of substrate fragments may conform to the principles of additivity.

RNase T2家族具有广泛的碱基特异性。RNase T2家族项目的目标是获得RNase T2家族酶的碱基识别和催化机制。RNase LE的底物-片段复合物显示出有趣的特征：底物片段（3′-GMP和5′-GMP）和底物类似物d（GpG）以生产性结合模式结合，而抑制剂（2′-GMP）以非生产性结合模式结合；3′-GMP在Bi亚位点和5′-GMP在B2亚位点的结合方式与d（GpG）在b1和B2亚位点的结合方式相似。这些观察结果表明，底物片段的结合可能符合可加性原理。

项目成果

T. Maruyama, S. Kozai, K. T. Nakamura, and M. Irie: "Synthesis of 2'-deoxy-2'-fluoroguanyl-(3', 5')-guanosine Nucleosides"Nucleotides and Nucleic Acids. (in press). (2002)

T. Maruyama、S. Kozai、K. T. Nakamura 和 M. Irie：“2-脱氧-2-氟鸟苷-(3, 5)-鸟苷核苷的合成”核苷酸和核酸。

桐野豊、中村和郎他: "生命薬学テキストシリーズ「物理化学(上)」" 共立出版, 約300頁 (1999)

Yutaka Kirino、Kazuo Nakamura 等人：“生物制药文本系列‘物理化学（第 1 部分）’”Kyoritsu Shuppan，约 300 页（1999 年）

Kiyoshi Ito: "The mechanism of substrate recognition of pyroglutamyl-peptidase I from Bacillus amyloliquefaciens as determined by X-ray Crystallography"Journal of Biological Chemistry. 276・21. 18557-18562 (2001)

Kiyoshi Ito：“通过 X 射线晶体学测定来自解淀粉芽孢杆菌的焦谷氨酰肽酶 I 的底物识别机制”《生物化学杂志》276·21（2001 年）。

Nobutada Tanaka: "Crystallization and Preliminary X-ray Crystallographic Studies of Ribonuclease LE from Lycopersicon Esculentum"Protein and Peptide Latters. 6・6. 407-410 (1999)

Nobutada Tanaka：“来自番茄的核糖核酸酶 LE 的结晶和初步 X 射线晶体学研究”蛋白质和肽后者 6・6（1999）。