The reaction mechanism of the ribonuclease T2 family

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The RNase T2 family shows broad base specificity. The objects of the project on the RNase T2 family are to gain the base-recognition and catalytic mechanism of the RNase T2 family enzymes. The substrate-fragment complexs of RNase LE reveal interesting features : the substrate fragments (3'-GMP and 5'-GMP) and substrate analogue d(GpG) bound in the productive binding mode while inhibitor (2'-GMP) bound in the non-productive binding mode ; and the binding mode of 3'-GMP at the Bi subsite and that of 5'-GMP at the B2 site are similar to that of d(GpG) bound at both B 1 and B2 subsite. These observations show that the binding of substrate fragments may conform to the principles of additivity.