The Reaction Mechanism and Subsites of RNase T_2 family

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During the present study we have successfully determined the two crucial crystal structures of RNase RNAP-Rh, i.e., RNase RNAP-Rh+d (ApG) complex and RNAP-Rh (Y57W) +d (ApC) complex. Diffraction data of the crystals were collected using an oscillation camera (R-AXISIIc manufactured by Rigaku). The crystal structures were determined based on the atomic coordinates of RNase Rh, and refined using the program X-PLOR.The refinements were done successfully, and the R-values of both complexes were coverged to under 0.20. (1) we found out that there are two hydrogen-bonds between the adenine base and the enzyme, and three ones between the phosphate group and the enzyme. (2) We also found out that the subsite B2 consists of Gln32, Pro92, Ser93, Asn94, Gln95 and Phe101.