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Real time imaging of neuronal excitation and cellular morphology of plastic synapses

神经元兴奋和塑料突触细胞形态的实时成像

基本信息

批准号：
10680741
负责人：
MURASE Kazuyuki
金额：
$ 2.5万
依托单位：
Fukui University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10680741/
关键词：
Synaptic Plasticity Long-term Potentiation Pain Spinal Cord Con-focal Microscope Optical Recording Slice Preparation Sensation レーザースキャン共焦点顕微鏡樹状突起蛍光染色 NMDA オピオイド

项目摘要

It is now believed that the long-term depression of synaptic transmissions from primary afferent C fibers to spinal dorsal horn neurons after conditioning low-frequency stimulation of A fibers take a critical role in the analgesia by adequate electrical or tactile stimulation. It is also known that dendritic morphology of some dorsal horn neurons is altered after the application of substance P, the pain transmitter/modulator released from pain-conducting primary afferent fibers. Therefore, it has been speculated that the morphological change might be caused by hyperalgesia and/or allodynia, and that it might be reset by analgesics such as morphine. The purpose of the present research is thus to reveal the properties of neuronal plasticity in the spinal dorsal horn. Especially, plastic changes in the neuronal excitation and neuronal morphology in the substantia gelatinosa of the dorsal horn are to be studied by using optical imaging methods with voltage sensitive dyes and con-focal micr … More oscope.We have revealed that conditioning high-frequency stimulation of A fibers in the dorsal root robustly changed C-fiber-evoked neuronal excitation, a facilitation under a low activity of inhibitory interneurons containing inhibitory amino acids, and suppression at the high activity. Conditioning low-frequency stimulation inhibited the induction and maintenance of the high-frequency conditioning-induced facilitation. Dendritic varicosities of some dorsal horn neurons in the gelatinosa, stained with DiI by the micro oil-drop method and observed with a laser-scan con-focal microscope, were expanded after the application of conditioning.These results thus exhibited clearly by optical imaging techniques, plastic changes in the dorsal horn, both in the signal transmission and in the neuronal morphology, take place by the conditioning stimulations known to induce hyperalgesia, allodynia or analgesia, and strongly indicated that such plasticity might be essential for the induction and transmission of the pain. Less

目前认为，A纤维低频刺激后，原传入C纤维向脊髓背角神经元突触传递的长期抑制在充分的电刺激或触觉刺激镇痛中起关键作用。我们也知道，一些背角神经元的树突形态在使用P物质后发生改变，P物质是从传导疼痛的初级传入纤维释放的疼痛递质/调节剂。因此，我们推测形态学改变可能是由痛觉过敏和/或异常性疼痛引起的，并可能通过吗啡等镇痛药物重置。因此，本研究的目的是揭示脊髓背角神经元可塑性的特性。利用电压敏感染料和共焦显微镜的光学成像技术，研究了大鼠背角明胶质的神经元兴奋和神经元形态的可塑性变化。我们发现，对背根A纤维的高频调节刺激可以显著改变c纤维引起的神经元兴奋，在含有抑制性氨基酸的抑制性中间神经元活性低时产生促进作用，在活性高时产生抑制作用。条件反射低频刺激抑制高频条件反射诱导的易化的诱导和维持。用DiI微油滴法染色，激光扫描共聚焦显微镜观察，经条件作用后，明胶水母部分背角神经元的树突状静脉曲张扩大。因此，这些结果通过光学成像技术清楚地显示，背角在信号传递和神经元形态方面的可塑性变化是由已知的诱发痛觉过敏、异常性疼痛或镇痛的条件刺激发生的，并强烈表明这种可塑性可能对疼痛的诱导和传递至关重要。少