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Imaging and Intracellular Analysis of Synaptic Plasticity in Spinal Dorsal Horn

脊髓背角突触可塑性的成像和细胞内分析

基本信息

批准号：
13680866
负责人：
MURASE Kazuyuki
金额：
$ 2.18万
依托单位：
Fukui University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13680866/
关键词：
Pain Optical imaging Spinal dorsal horn Presynaptic modulation スライスパッチ

项目摘要

1. Imaging of voltage response of marginal cell in the lamina I of the dorsal horn retrogradely stained with voltage-sensitive dye.Projection neurons in the lamina I were stained by voltage-sensitive dye that was injected in the thalamus or brain stem. After one week of the dye injection, slices of the spinal cord were made and the stained cells were recorded by an optical imaging method. The electrical activities evoked by dorsal root stimulation was successfully recorded optically with high resolution in both time and space. The action potentials and the after potentials were clearly observed. We are currently investigating the underlying mechanism of de-potentiation of LTP with this preparation.2. Modulation of dorsal horn plasticity induction by corticotropin releasing factor (CRF)We have searched substances that regulate induction of dorsal plasticity, which takes an important role in the induction of abnormal pain. We have found that CRF biphasically regulates the induction of robust changes in neuronal excitation in the superficial dorsal horn by optical imaging.3. Optical analysis of changes in extracellular space : Possible role for non-synaptic transmission.Intrinsic optical signals (IOS) in slice has been suggested to reflect cellular volume or extracellular space. We have revealed that cotransporters and water channels play the major role for the IOS induction in the superficial dorsal horn. We are currently investigating on their modulators and the changes in electrical activities associated with the volume changes by optical imaging.4. Optical analysis of presynaptic modulation at the primary afferent terminals.By optical imaging, we were able to record presynaptic events, and we investigated on the modulators. We analysed the presynaptic actions of halothane and caffeine as well as vanilloid receptor agoninsts.

1.背角I层边缘细胞电压反应的逆行染色：用注射于丘脑或脑干的电压敏感染料对I层投射神经元进行染色。注射染料1周后，制作脊髓切片，用光学成像方法记录染色细胞。用光学方法成功地记录了刺激背根诱发的电活动，具有较高的时间和空间分辨率。动作电位和后电位被清晰地观察到。我们目前正在用这种制备方法研究LTP去增强的潜在机制。促肾上腺皮质激素释放因子(CRF)对背角可塑性诱导的调节作用我们已经找到了调节背角可塑性诱导的物质，它在异常疼痛的诱导中起着重要作用。我们通过光学成像发现，CRF对浅背角神经元兴奋的诱导具有双相调节作用。细胞外间隙变化的光学分析：非突触传递的可能作用。切片中的固有光信号(IO)被认为是反映细胞体积或细胞外间隙的指标。我们发现，在背角浅层，共转运蛋白和水通道在IOS的诱导中起主要作用。我们目前正在通过光学成像研究它们的调制器以及与体积变化相关的电活动的变化。初级传入终末突触前调制的光学分析通过光学成像，我们能够记录突触前事件，并对调制器进行了研究。我们分析了氟烷、咖啡因以及香草素受体激动剂的突触前作用。