Silent synapses and long-term potentiation in the spinal cord analyzed by imaging of pre- and postsynaptic excitation

通过突触前和突触后兴奋成像分析脊髓中的沉默突触和长时程增强

• 批准号: 16500262
• 负责人: MURASE Kazuyuki
• 金额: $ 2.43万
• 依托单位: University of Fukui
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16500262/
• 关键词: synapse; imaging; nitric oxide; voltage-sensitive dye; spinal cord; pain; バニロイド; カプサイシン

Pain-conducting primary afferent fibers terminate in superficial parts of the spinal dorsal horn. The afferent synapses are plastic and long-term potentiation of the synaptic efficacy is considered to be responsible for the induction of the Hyperalgesia at least in part. We have investigated the plasticity by imaging neuronal excitation in spinal cord slices stained with voltage dye. Recently we succeeded to image more specifically the excitation of pre and postsynaptic elements, namely (1) retrograde staining of afferent fibers to visualize presynaptic events, and (2) anterograde staining of projection neurons in the superficial dorsal horn to visualize postsynaptic events. In preliminary experiments, we observed two interesting events. One is that after conditioning stimulation, silent synapses became active and also silent afferent terminals started generating action potentials. Another is that nitric oxide (NO) contributed to the induction.In this study, we first investigated on th … More e mechanism how the silent synapses become active by using the retrograde and anterograde staining of pre and postsynaptic elements, respectively. We have confirmed that after conditioning, presynaptic terminals started generating action potentials and thus silent synapses became active. Secondly, we tried to reveal the signaling pathway through which NO induced the potentiation by using double staining of slice with voltage dye and NO-sensitive dye. We have shown that the amount of NO released after conditioning is strongly correlated with the degree of long-term potentiation. In addition, we have shown that, after the treatment with a glial metabolism inhibitor, both induction of long-term potentiation and NO release did not take place, results suggesting glial cells contribute for the NO release. Thirdly, we investigated the mechanism how glial cells contribute to the long-term potentiation, especially whether or not ATP contribute to it. We have obtained results showing that neuron-glia interactions via P2X receptors of ATP is essential for the induction of long-term potentiation. Fourthly, we have revealed the mechanism of presynaptic inhibition via presynaptic vanilloid receptors that is ATP- and temperature dependent. Less

支配痛觉的初级传入纤维终止于脊髓背角的浅部。传入突触是可塑性的，突触效能的长期增强被认为至少部分地导致了痛觉过敏的诱导。我们用电压染料染色的脊髓切片进行神经兴奋成像，研究了脊髓切片的可塑性。最近，我们成功地对突触前和突触后成分的兴奋进行了更准确的成像，即(1)逆行染色传入纤维以显示突触前事件，(2)顺行染色背角浅层投射神经元以显示突触后事件。在初步实验中，我们观察到了两个有趣的事件。一种是在条件性刺激后，沉默的突触变得活跃，沉默的传入终末也开始产生动作电位。另一种是一氧化氮(NO)参与了诱导。在本研究中，我们首先研究了…通过对突触前成分的逆行染色和突触后成分的顺行染色，进一步阐明了沉默突触是如何变得活跃的。我们已经证实，在条件作用后，突触前终末开始产生动作电位，从而使沉默的突触变得活跃。其次，我们用电压染料和非敏感染料双重染色的方法，试图揭示NO诱导增强的信号通路。我们已经证明，条件反射后释放的NO的量与长时程增强的程度密切相关。此外，我们还发现，在胶质代谢抑制剂处理后，长时程增强的诱导和NO的释放都没有发生，这一结果表明胶质细胞参与了NO的释放。第三，我们研究了神经胶质细胞对长时程增强的作用机制，特别是三磷酸腺苷是否对其有贡献。我们已经获得的结果表明，通过ATP的P2X受体的神经元-胶质细胞相互作用对于诱导长时程增强是必不可少的。第四，我们揭示了通过突触前香草素受体抑制突触前的机制，这种机制是依赖于ATP和温度的。较少

项目成果

Parallel Cooperative Neural Controller Evolution for Autonomous Robots in a Real Environment

真实环境中自主机器人的并行协作神经控制器进化

• 发表时间: 2005
• 期刊: WSEAS Trans Information Science and Applications Vol.2, No.10
• 作者: Md.Shahjahan;Yukichika Sakuragawa;Kazuyuki Murase
• 通讯作者: Kazuyuki Murase

A Neural Network Training Algorithm with Positive Correlation

一种正相关神经网络训练算法

• 发表时间: 2005
• 期刊: IEICE Trans Information and Systems Vol.E88-D, No.10
• 作者: Md.Shahjahan;Kazuyuki Murase
• 通讯作者: Kazuyuki Murase

Nitric Oxide-Dependent Long-Term Potentiation Revealed by Real Time Imaging of Nitric Oxide Production and Neuronal Excitation in the Dorsal Horn of Rat Spinal Cord Slices

大鼠脊髓切片背角一氧化氮产生和神经元兴奋的实时成像揭示一氧化氮依赖性长时程增强

• 期刊: European Journal of Neuroscience (in press)
• 作者: Ikeda H;Kusudo K;Murase K
• 通讯作者: Murase K

Chaotic dynamics of a behavior-based miniature mobile robot: effects of environment and control structure

• DOI: 10.1016/j.neunet.2004.09.002
• 发表时间: 2005-03
• 期刊: Neural networks : the official journal of the International Neural Network Society
• 作者: M. Islam;K. Murase

Depression of presynaptic excitation by the activation of vanilloid receptor 1 in the rat spinal dorsal horn revealed by optical imaging

光学成像揭示大鼠脊髓背角香草素受体 1 的激活抑制突触前兴奋

• 发表时间: 2006
• 期刊: Molecular Pain 2:8
• 作者: Kei Kusudo;Hiroshi Ikeda;Kazuyuki Murase
• 通讯作者: Kazuyuki Murase