Development of selective, rapid and simple sensing system for oraganohalogen Compound

最近，二恶英和双酚A等内分泌干扰物造成的环境污染在日本正成为一个严重的问题。然而，由于没有简单、快速的内分泌干扰物测定方法的报道，没有对现场环境污染的真实情况进行充分的检查。内分泌干扰物的测定通常采用气相色谱-质谱法(GC-MS)。GC-MS测定内分泌干扰物的过程非常耗时，需要非常熟练的技术。因此，迫切需要一种方便、简单、快速的分析方法来检测和测量环境领域中的内分泌干扰物。本课题利用线阵电荷耦合器件研制了一种便携式便携式SPR传感器，用于检测传感器的反射光。研制的SRR传感器的尺寸约为8 cm×6 cm×4.8 cm。传感器的重量小于1公斤。以邻苯二甲酸二丁酯和磷酸三氯二酯为原料，按1：1的重量比制成增塑聚氯乙烯薄膜，在棱镜和传感器芯片之间建立了一种新的光接口，制备的聚氯乙烯薄膜的折射率为1.561。这种膜的折射率与传统的配对油相同。研制了一种用于便携式SPR传感器的传感器芯片。传感器芯片为2厘米高的圆柱形试管，玻璃上贴有一层薄薄的金膜。匹配膜对传感器芯片的粘附性很强，适合粘贴在便携式SPR传感器上。所研制的SPR传感器已成功应用于2，4-二氯苯酚的测定，检测精度可达0.1ppm

Recently, environmental pollution due to endocrine disruptors such as dioxin and bis-phenol A is becoming a serious problem in Japan. However, the real state of the environmental pollution in field is not examined adequately because no simple and rapid analytical methods for the determination of endocrine disruptors were reported. The determination of endocrine disruptors is usually determined by gas chromatography-mass spectrometry (GC-MS). The procedure of GC-MS for the determination of endocrine disruptors is time-consuming and needs a very skilled technique. As a result, urgent needs have been developed for potable, simple and rapid analytical methods for the detection and measurement of endocrine disruptors in environmental field. In this project, a portable and handy-type SPR sensor was developed by using a line CCD for detection of reflected light of the sensor. The dimension of the developed SRR sensor was about 8 cm x 6 cm x 4.8 cm. The weight of the sensor was less than 1 kg. A new light interface between the prism and the sensor chip was developed by using a plasticized PVC film, which was prepared from dibutylphthalate and tricrediyl phosphate at the weight ratio of 1 : 1. The refractive index of the prepared PVC film was 1.561. This film has the same refractive index as of the conventional mating oil. A sensor chip for the portable SPR sensor was newly developed. The sensor chips was cylidrical cuvette with 2 cm-hight, and a thin gold film on the glass was attached at the end of the cuvette. The matching film, which was very sticky to the sensor chip, was suitable for attaching on the portable SPR sensor. The developed SPR sensor was successfully applied to the determination of 2,4-dichlorophenol down to 0.1 ppm level