Development technique of bone regeneration using Mesenchymal Stem Cell
利用间充质干细胞进行骨再生的开发技术
基本信息
- 批准号:11307048
- 负责人:
- 金额:$ 24.47万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (A)
- 财政年份:1999
- 资助国家:日本
- 起止时间:1999 至 2001
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Recent studies in the field of tissue engineering have developed and established the technique of cultured bone regeneration with mesenchymal stem cells (MSCs) derived from bone marrow. Most of characteristics of the regenerat ion have been clarified by these recent studies. Generally, it have described a new technology called tissue engin eering that involves the morphogenesis of new tissue from constructs formed of isolated cells, biocompatible scaff olds and growth factors. In the present studies, we used MSCs as cell sources, ceramics and absorbable collagen as matrices, and rhBMP-2 and Platelet Rich Plasma as growth factors. In order to examine the effectiveness of cultur ed bone regeneration for clinical use, we investigated the following five things : 1) development of matrix, 2) ost eogenesis in vivo, 3) establishment of the method of larger scale of osteoblast culture, 4) the effectiveness of growth factors to improve the bone formation. 5) A possibility of clinical application in human. In regard to the matrix, we investigated the osteogenic potential of non resorbable HA and a new biodegradable β-tricalcium phosph ate (β-TCP), showing that β-TCP would be an ideal scaffolds for bone regeneration. In addition, we found that absorbable collagen would be an effective scaffold for rhBMP-2 in the sinus as well as autogenous bone. However, m ost studies regarding bone formation have adopted two dimensional histomorphometric evaluations and molecular biol ogys, not three dimensional such as micro CT. We also successfully evaluated microstructure of trabecular bone in human clinical cases. In some years from now, we are going to perform studies to improve the utility of the graft for the periodontal or alveolar defect for clinical use of technique of the cultured bone regeneration using MSCs with plasticity.
近年来,组织工程领域的研究发展并确立了骨髓间充质干细胞(MSCs)体外培养成骨的技术。这些最新的研究已经阐明了再生的大部分特征。一般说来,它描述了一种名为组织工程的新技术,该技术涉及从分离的细胞、生物兼容的细胞和生长因子形成的结构中形成新组织的形态形成。本研究以骨髓间充质干细胞为细胞来源,以陶瓷和可吸收胶原为基质,以重组人骨形态发生蛋白-2和富血小板血浆为生长因子。为了检验体外培养骨再生的临床应用效果,我们从以下五个方面进行了研究:1)基质的发育;2)体内成骨细胞的形成;3)成骨细胞大规模培养方法的建立;4)生长因子促进骨形成的效果。5)临床应用的可能性。在基质方面,我们研究了不可吸收羟基磷灰石和一种新型的可生物降解的β-磷酸三钙(β-TcP)的成骨能力,表明β-TcP是一种理想的骨再生支架。此外,我们还发现可吸收胶原在鼻窦和自体骨中可以作为重组人骨形态发生蛋白-2的有效支架。然而,大多数关于骨形成的研究采用了二维组织形态计量学和分子生物学方法,而不是像Micro CT这样的三维空间。我们还成功地在人类临床病例中评估了松质骨的微结构。在未来几年内,我们将开展研究,以提高牙周或牙槽骨缺损的移植物的实用性,为具有可塑性的MSCs培养骨再生技术的临床应用奠定基础。
项目成果
期刊论文数量(41)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Minoru Ueda: "Tissue Engineering in Bone and Cartilage"Transplant Today. 14.5. 612-618 (2001)
Minoru Ueda:“骨和软骨的组织工程”今日移植。
- DOI:
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- 影响因子:0
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- 通讯作者:
Minoru Ueda: "Hard and soft tissue engineering: applications in implant surgery"Tissues Engineering for Therapeu tic. 191-202 (2001)
Minoru Ueda:“硬组织工程和软组织工程:在种植手术中的应用”用于治疗的组织工程。
- DOI:
- 发表时间:
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- 影响因子:0
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- 通讯作者:
上田 実: "ティッシュエンジニアリングの最新動向-幹細胞を利用した骨再生"The Quintessence Year Book 2001. 10-14 (2001)
Minoru Ueda:“组织工程的最新趋势 - 使用干细胞进行骨再生”The Quintessence Year Book 2001. 10-14 (2001)
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Masaki Honda: "Cartilage Formation by Cultured Chondrocytes in a New Scaffold Made of Poly(I,-I, actide-ε-caprolaotone) sponge"Journal of Oral & Maxillofacial Surgely. 58. 767-775 (2000)
Masaki Honda:“由聚(I,-I,actide-ε-己内酯)海绵制成的新支架中培养的软骨细胞的软骨形成”口腔颌面外科杂志 58. 767-775(2000)。
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- 影响因子:0
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UEDA Minoru其他文献
Improvement of High Temperature Fatigue Properties of TiAl Alloys Fabricated by Electron Beam Melting Through Hot Isostatic Pressing Process
热等静压电子束熔炼TiAl合金高温疲劳性能的改善
- DOI:
10.7791/jspmee.9.180 - 发表时间:
2020 - 期刊:
- 影响因子:0
- 作者:
CHO Ken;YASUDA Hiroyuki;TODAI Mitsuharu;UEDA Minoru;TAKEYAMA Masao;NAKANO Takayoshi - 通讯作者:
NAKANO Takayoshi
高クロム系多合金白鋳鉄の高温エロージョン摩耗特性に及ぼすチタン添加の影響
添加钛对高铬多元合金白口铸铁高温冲蚀磨损特性的影响
- DOI:
- 发表时间:
2022 - 期刊:
- 影响因子:0
- 作者:
CHO Ken;YASUDA Hiroyuki;TODAI Mitsuharu;UEDA Minoru;TAKEYAMA Masao;NAKANO Takayoshi;門間雄大,清水一道,楠本賢太 - 通讯作者:
門間雄大,清水一道,楠本賢太
UEDA Minoru的其他文献
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{{ truncateString('UEDA Minoru', 18)}}的其他基金
Regulation of plant hormone receptor using stereochemical isomers of a natural product
利用天然产物的立体化学异构体调节植物激素受体
- 批准号:
18KK0162 - 财政年份:2019
- 资助金额:
$ 24.47万 - 项目类别:
Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))
Molecular mechanism of trap-snapping movement of carnivorous plant
食虫植物捕捉运动的分子机制
- 批准号:
24651236 - 财政年份:2012
- 资助金额:
$ 24.47万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Development of new regenerative therapy for central nerve system using the conditioned media derived from human dental pulp stem cells
使用源自人牙髓干细胞的条件培养基开发新的中枢神经系统再生疗法
- 批准号:
23659914 - 财政年份:2011
- 资助金额:
$ 24.47万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Intracellular glycoside signal participating in the regulation of biological phenomena
细胞内糖苷信号参与生物现象的调节
- 批准号:
23310147 - 财政年份:2011
- 资助金额:
$ 24.47万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Analysis of benefits of dental pulp stem cells from permanent and milk teeth for the treatment of the intractable diseases and of their banking system.
分析恒牙和乳牙牙髓干细胞治疗疑难杂症及其银行系统的益处。
- 批准号:
21390524 - 财政年份:2009
- 资助金额:
$ 24.47万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Bioorganic studies on membrane protein involved in the regulation of plant ion channels
参与植物离子通道调节的膜蛋白的生物有机研究
- 批准号:
20310128 - 财政年份:2008
- 资助金额:
$ 24.47万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Periodontal tissue regeneration method by regenerative medicine with stem cells-multi-center study for practical use
干细胞再生医学牙周组织再生方法-多中心实用化研究
- 批准号:
16390583 - 财政年份:2004
- 资助金额:
$ 24.47万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Experimental study of heterotopic bone formation by in vivo and in vitro gene transfer of bone morphogenetic protein(BMP) and sonic hedgehog(SHH) in the maxillofacial resions.
颌面部骨形态发生蛋白(BMP)和音刺猬(SHH)体内外基因转移异位骨形成的实验研究。
- 批准号:
13470430 - 财政年份:2001
- 资助金额:
$ 24.47万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Study of cultured bone or cartilage regeneration using human mesenchymal stem cells
利用人间充质干细胞培养骨或软骨再生的研究
- 批准号:
12794015 - 财政年份:2000
- 资助金额:
$ 24.47万 - 项目类别:
Grant-in-Aid for University and Society Collaboration
Bioorganic studies on nyctinastic leaf-movement controlled by a biological clock
生物钟控制的夜间叶运动的生物有机研究
- 批准号:
12680598 - 财政年份:2000
- 资助金额:
$ 24.47万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
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