Study of cultured bone or cartilage regeneration using human mesenchymal stem cells

利用人间充质干细胞培养骨或软骨再生的研究

• 批准号: 12794015
• 负责人: UEDA Minoru
• 金额: $ 23.68万
• 依托单位: Nagoya Univeristy
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for University and Society Collaboration
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12794015/
• 关键词: Cultured Bone; Cultured cartilage; graft materials; Mechanical Stress; Mesenchymal Stem Cells; Culture on a large scale; Cultured periosteum; ティッシュエンジニアリング; 大量培養法; ヒト

Recently, it is obvious that mesenchymal stem cells (MSCs) differentiate various human tissues and these cells proliferation technology comes true. On the other hand, biodegradable matrix with differentiation and proliferation of the cells developed by rapid development of matrix engineering. On the basis of these technology, tissue engineering, which involves the morphogenesis of new tissue from constructs formed of isolated cells, biocompatible scaffolds and growth factor, was the focus of attention. By using this method, we can regenerate a living tissue using patioents' self. These can cure the difficult treatment of chronic bone and joint disease. At first, we pay attention to develop graft materials as a matrix and improve calcium phosphate. It called CPC and was compared to β-TCP. And in animal study with osteogenesis, it was clear that the materials had good ability of bone regeneration. Therefore we can expect application of the bone materials. And applying fibrin glue and platelet rich plasma, we can confirm that these material have ability of good bone regeneration. As for cell culture method, we can get acceleration of the cell function using stress, such as stretch stress, pressure stress and supersonic waves and apply bone regeneration. There were not big differences in function of chondrocytes, which differentiated from MSCs, using the same stress. And as for culture method on a large scale using a bioreactor, it was useful under using pressure stress. In the future, by applying these results, we would like to suggest cell providing method in regenerating bone and cartilage using MSCs and strategy of fostering new industry.

最近，很明显，间充质干细胞（MSC）区分了各种人体组织，这些细胞增殖技术成立。另一方面，可生物降解的基质具有分化和通过基质工程快速发展而开发的细胞增殖。根据这些技术，组织工程涉及由孤立细胞形成的构建体，生物相容性支架和生长因子的新组织的形态发生，这是关注的重点。通过使用这种方法，我们可以使用受试者的自我再生生物组织。这些可以治愈慢性骨和关节疾病的困难治疗。首先，我们注意将级材料作为基质开发，并改善磷酸钙。它称为CPC，并与β-TCP进行了比较。在动物发生的动物研究中，很明显材料具有良好的骨再生能力。因此，我们可以预期应用骨料。并应用纤维蛋白胶和富含血小板的血浆，我们可以确认这些材料具有良好的骨再生能力。至于细胞培养方法，我们可以使用应力（例如拉伸应力，压力应力和超音速波）来加速细胞功能，并施加骨再生。使用相同的应力，在与MSC区分的软骨细胞功能上没有很大的差异。至于使用生物反应器的大规模培养方法，在使用压力应力下，它很有用。将来，通过应用这些结果，我们想建议使用MSC和促进新工业的策略来再生骨骼和软骨的细胞。

项目成果

Minoru Ueda: "The potential of Regenerative medicine changing the dental therapy in 21 century"Journal of The Association for Tokyo Dental Doctor. 49-2. 47-59 (2001)

Minoru Ueda：“再生医学改变 21 世纪牙科治疗的潜力”东京牙科医师协会杂志。

Megmi Matsuno: "In vitoro Analysis Distraction osteogenesis"The Journal of Craniofacial Surgery. 11-4. 303-307 (2000)

Megmi Matsuno：“体外分析牵引成骨”颅面外科杂志。

上田 実: "これからの歯科は幹細胞の時代"The Quintessence. 21・1. 47-54 (2002)

Minoru Ueda：“牙科的未来是干细胞的时代”The Quintessence 21・1（2002）。

Minoru Ueda: "Tissue engineering in research in oral implant surgery"Artificial Organs. 25・3. 164-171 (2001)

Minoru Ueda：“口腔种植外科研究中的组织工程”Artificial Organs 164-171（2001）。

Yoichi Yamada: "Injectable Bone"Clinical Calcium. 12-2. 50-54 (2002)

山田洋一：“可注射骨”临床钙。