Study of cultured bone or cartilage regeneration using human mesenchymal stem cells

利用人间充质干细胞培养骨或软骨再生的研究

• 批准号: 12794015
• 负责人: UEDA Minoru
• 金额: $ 23.68万
• 依托单位: Nagoya Univeristy
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for University and Society Collaboration
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12794015/
• 关键词: Cultured Bone; Cultured cartilage; graft materials; Mechanical Stress; Mesenchymal Stem Cells; Culture on a large scale; Cultured periosteum; ティッシュエンジニアリング; 大量培養法; ヒト

Recently, it is obvious that mesenchymal stem cells (MSCs) differentiate various human tissues and these cells proliferation technology comes true. On the other hand, biodegradable matrix with differentiation and proliferation of the cells developed by rapid development of matrix engineering. On the basis of these technology, tissue engineering, which involves the morphogenesis of new tissue from constructs formed of isolated cells, biocompatible scaffolds and growth factor, was the focus of attention. By using this method, we can regenerate a living tissue using patioents' self. These can cure the difficult treatment of chronic bone and joint disease. At first, we pay attention to develop graft materials as a matrix and improve calcium phosphate. It called CPC and was compared to β-TCP. And in animal study with osteogenesis, it was clear that the materials had good ability of bone regeneration. Therefore we can expect application of the bone materials. And applying fibrin glue and platelet rich plasma, we can confirm that these material have ability of good bone regeneration. As for cell culture method, we can get acceleration of the cell function using stress, such as stretch stress, pressure stress and supersonic waves and apply bone regeneration. There were not big differences in function of chondrocytes, which differentiated from MSCs, using the same stress. And as for culture method on a large scale using a bioreactor, it was useful under using pressure stress. In the future, by applying these results, we would like to suggest cell providing method in regenerating bone and cartilage using MSCs and strategy of fostering new industry.

近年来，间充质干细胞明显分化为多种人体组织，并实现了这些细胞的增殖技术。另一方面，随着基质工程的快速发展，具有分化和增殖能力的生物可降解基质材料得到了迅速发展。在这些技术的基础上，组织工程成为人们关注的焦点，组织工程涉及从分离细胞、生物相容性支架和生长因子形成的结构中形成新组织的形态形成。通过这种方法，我们可以利用患者的自身再生出活组织。这些都可以治愈慢性骨关节疾病的治疗难题。首先，我们注意开发接枝材料作为基质，并对磷酸钙进行改进。它被称为CPC，并与β-Tcp进行了比较。动物成骨实验表明，该材料具有良好的成骨能力。因此，我们可以期待这种骨材料的应用。应用纤维蛋白胶和富血小板血浆，可以证实这些材料具有良好的骨再生能力。至于细胞培养方法，我们可以利用应力，如拉伸应力、压力应力和超声波等来加速细胞的功能，并应用于骨再生。在相同的应力下，从MSCs分化而来的软骨细胞在功能上没有太大差异。而采用生物反应器大规模培养的方法，在压力胁迫下是可行的。未来，通过应用这些结果，我们将提出利用MSCs提供细胞再生骨和软骨的方法和培育新产业的策略。

项目成果

Minoru Ueda: "Tissue engineering in research in oral implant surgery"Artificial Organs. 25・3. 164-171 (2001)

Minoru Ueda：“口腔种植外科研究中的组织工程”Artificial Organs 164-171（2001）。

上田 実: "これからの歯科は幹細胞の時代"The Quintessence. 21・1. 47-54 (2002)

Minoru Ueda：“牙科的未来是干细胞的时代”The Quintessence 21・1（2002）。

Megmi Matsuno: "In vitoro Analysis Distraction osteogenesis"The Journal of Craniofacial Surgery. 11-4. 303-307 (2000)

Megmi Matsuno：“体外分析牵引成骨”颅面外科杂志。

Minoru Ueda: "The potential of Regenerative medicine changing the dental therapy in 21 century"Journal of The Association for Tokyo Dental Doctor. 49-2. 47-59 (2001)

Minoru Ueda：“再生医学改变 21 世纪牙科治疗的潜力”东京牙科医师协会杂志。

Minoru Ueda: "Tissue engineering : applications for maxillofacial surgery"Materials Science and Engineering. 13. 7-14 (2000)

Minoru Ueda：“组织工程：颌面外科的应用”材料科学与工程。