Bioorganic studies on nyctinastic leaf-movement controlled by a biological clock

生物钟控制的夜间叶运动的生物有机研究

• 批准号: 12680598
• 负责人: UEDA Minoru
• 金额: $ 2.56万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12680598/
• 关键词: legumes; nyctinasty; leaf-opening substance; β-glucosidase; inhibitor; マメ科植物; 光親和性プローブ; ネムノキ; カワラケツメイ; マメ科; 除草剤; 蛍光プローブ化合物; 水分蒸散; クチクラ蒸散; ギンネム

Most leguminous plants close their leaves in the evening, as if to sleep, and open them early in the morning. This circadian rhythm is known to be controlled by a biological clock of such plants. Extensive studies on some nyctinastic plants led to the isolation of a variety of leaf-closing and leaf-opening substances. And we found that a biological clock regulates the balance of concentration between leaf-opening and -closing substances in the plant body through the activation of β-glucosidase and generates the circadian rhythm of leaf-movement (Figure). Also, we will show two examples of our attempts for the bioorganic studies of plant leaf-movement using synthetic probe compounds. One is the direct observation of the target cell for leaf-movement factor by fluorescence-labeled probe compounds and the other is the chemical study on the historical problem, "Why does the plant sleep?" by using synthetic non-natural natural products that inhibit the leaf closure.

大多数豆科植物在傍晚关闭叶子，好像要睡觉，并在清晨打开它们。已知这种昼夜节律由此类植物的生物时钟控制。对某些杂种植物的广泛研究导致分离了各种叶片叶片和叶片叶片。而且我们发现，生物时钟通过激活β-葡萄糖苷酶的激活来调节植物体中叶片和裂入物质之间的浓度平衡，并产生叶片移动的昼夜节律（图）。此外，我们将展示我们使用合成探针化合物进行植物叶片运动生物有机研究尝试的两个例子。一种是通过荧光标记的探针化合物对目标细胞的直接观察到叶子运动因子，另一个是关于历史问题的化学研究，“植物为什么睡觉？”通过使用抑制叶片闭合的合成非天然产物。

项目成果

上田実: "Potassium 2, 3, 4-trihydroxy-2-methylbutanoate, a leaf-closing substance of Leucaena Leucocephalam "Tetrahedron Letters. 42(in press). (2001)

Minoru Ueda：“2,3,4-三羟基-2-甲基丁酸钾，银合欢的闭叶物质”Tetrahedron Letters 42（出版中）。

上田実: "Synthetic probe compounds for bioorganic studies of hyctinasty"Tetrahedron. 56. 5123-5130 (2000)

Minoru Ueda：“用于 hyctinasty 生物有机研究的合成探针化合物”Tetrahedron 56. 5123-5130 (2000)。

T.Fujii et al.: "Synthesis of a novel bioactive photoaffinity probe based on a leaf-movement factor with potential high biudiy affinity"Tetrahedron Letters. 44. 2497-2499 (2003)

T.Fujii 等人：“基于具有潜在高双亲和力的叶运动因子的新型生物活性光亲和探针的合成”Tetrahedron Letters。

T.Sugimoto: "Syntheses of a novel photoaffinity probes for bioorganic studies on nyctinasty"Tetrahedron Letters. 43. 6529-6532 (2002)

T.Sugimoto：“用于夜间生物有机研究的新型光亲和探针的合成”四面体快报。

N.Takada, E.Kato, K.Ueda, S.Yamamura, M.Ueda: "A Novel Leaf-movement Inhibitor of a Nyctinastic Weed Sesbania exaltata Cory, Designed on a Naturally Occurring Leaf-opening Substance and its Application for Potential Highly Selective Herbicide"Tetrahedron

N.Takada、E.Kato、K.Ueda、S.Yamamura、M.Ueda：“一种新型催眠杂草田菁叶运动抑制剂，基于天然存在的叶开放物质设计及其在潜在高度开放中的应用