Studies on super sensitive analysis with atto mole order by means of capillary electrophoresis equipped with chemiluminescence detection. -Development of high performance chemiluminescence detection cell using an optical fiber-

毛细管电泳结合化学发光检测阿托摩尔级超灵敏分析研究。

• 批准号: 11650837
• 负责人: TSUKAGOSHI Kazuhiko
• 金额: $ 0.96万
• 依托单位: Doshisha University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11650837/
• 关键词: Capillary electrophoresis; Chemiluminescence; Sensitive analysis; Luminol; Optical fiber; Separation detection; キャピラリー電気泳動; 高感度

A new and simple chemiluminescence detection cell using optical fiber was designed for capillary electrophoresis. The cell was easily combined with capillary electrophoresis and the system was operated without any tedious procedures. Luminol chemiluminescence was adapted for use with this detection cell. Hydrogen peroxide and catalysts were in detail examined concerning their usage and concentration with the cell. The detection limit for luminol was 5.0 x 10^<-10> M (14 amol ; S/N = 3), which was the most sensitive result reported so far. Also, a mixture sample of glycine, glycylglycine, and glycylglycylglycine which were labeled with isoluminol isothiocyanate was subjected to the present system. They were sensitively detected and represented with complete base-line separations.The concept of chemiluminescence detection cell using optical fiber was applied for the following researches : "Chemiluminescence detection of heme proteins separated by capillary isoelectric focusing", "Batch-type detection cell using a peroxyoxalate chemiluminescence system for capillary electrophoresis", "Design of pressure-mobilization system for capillary isoelectric focusing - chemiluminescence detection", and "Flow-type chemiluminescence detection cell using optical fiber for capillary electrophoresis".

设计了一种新型的、结构简单的光纤毛细管电泳化学发光检测池。该池很容易与毛细管电泳结合，系统操作无需任何繁琐的程序。鲁米诺化学发光适用于该检测池。详细检查过氧化氢和催化剂的使用情况和在电池中的浓度。鲁米诺的检测限为5.0 × 10^<-10>M（14阿莫尔; S/N = 3），这是迄今为止报道的最灵敏的结果。此外，将用异鲁米诺异硫氰酸酯标记的甘氨酸、甘氨酰甘氨酸和甘氨酰甘氨酰甘氨酸的混合物样品置于本系统中。它们被灵敏地检测到并以完全基线分离表示。使用光纤的化学发光检测池的概念被应用于以下研究：“毛细管等电聚焦分离血红素蛋白的化学发光检测”，“使用过氧草酸盐化学发光体系进行毛细管电泳的批式检测池”，“毛细管等电聚焦-化学发光检测压力动员系统的设计”和“毛细管电泳光纤流动式化学发光检测池”。

项目成果

K.Tsukagoshi: "Improvement of a Capillary Electrophoresis-Chemiluminescence Detection System for Using a Polyacrylamide-Coated Capillary"Anal.Sci.. 17. 345-347 (2001)

K.Tsukagoshi：“使用聚丙烯酰胺涂层毛细管的毛细管电泳-化学发光检测系统的改进”Anal.Sci.. 17. 345-347 (2001)

M.Hashimoto: "Compact detection cell using optical fiber for sensitization and simplification of capillary electrophoresis-chemiluminescence detector"Journal of Chromatography A. 832. 191-202 (1999)

M.Hashimoto：“使用光纤对毛细管电泳-化学发光检测器进行敏化和简化的紧凑型检测池”《色谱杂志》A. 832. 191-202 (1999)

Masahiko Hashimoto, Kazuhiko Tsukagoshi, Riichiro Nakajima, and Kazuo Kondo: "Compact Detection Cell Using Optical Fiber for Sensitization and Simplification of Capillary Electrophoresis - Chemiluminescence Detector"Journal of Chromatography A. 832. 191-2

Masahiko Hashimoto、Kazuhiko Tsukagoshi、Riichiro Nakajima 和 Kazuo Kondo：“使用光纤实现毛细管电泳敏化和简化的紧凑型检测单元 - 化学发光检测器”色谱杂志 A. 832. 191-2

M.Hashimoto: "Flow-type Chemiluminescence Detectiion Cell Using Optical Fiber for Capillary Electrophoresis"Bulletin of the Chemical Society of Japan. 72. 2673-2679 (1999)

M.Hashimoto：“使用光纤进行毛细管电泳的流式化学发光检测池”日本化学会会刊。

M.Hashimoto: "Flow-type Chemilaminescerce Detection Cell Using Opticall Fiber for Capillary Electrophoresis"Eull.Chem.Sec.Jpn.. 72. 2673-2679 (1999)

M.Hashimoto：“使用光纤进行毛细管电泳的流动型化学胺检测池”Eull.Chem.Sec.Jpn.. 72. 2673-2679 (1999)