Studies on Chemiluminescence Analysis Using Liquid-Liquid Micro-Space Interface in a Micro-Channel

微通道液-液微空间界面化学发光分析研究

• 批准号: 20550087
• 负责人: TSUKAGOSHI Kazuhiko
• 金额: $ 3.08万
• 依托单位: Doshisha University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20550087/
• 关键词: 微小空間; 層流; マイクロチャネル化学発光分析; 管径方向分配クロマトグラフィー; マイクロチャネル; 化学発光; 液-液界面; マイクロフロー; 管径方向分配; マイクロチャンネル; 過シュウ酸エステル

A novel chemiluminescence analysis to taked advantage of a specific chemiluminescence reaction space around the liquid-liquid interface in a micro-channel under laminar flow conditions has been reported. We call the analytical system "micro-channel chemiluminescence analysis (MCCLA)". First, the concept of micro-channel chemiluminescence analysis and its features were described and considered through direct observations of fluorescence and chemiluminescence using a fluorescence microscope-CCD camera and a microscope CCD camera. Next, several concrete examples of micro-channel chemiluminescence analysis were introduced, and the experimental data were basically discussed. A fluorescence compound was examined by micro-channel chemiluminescence analysis using an oxalate chemiluminescence reaction. Anti-oxygen, beverages, and saliva were also investigated by chemiluminescence analysis using a singlet oxygen chemiluminescence reaction. Furthermore, we have developed a capillary chromatography using an open capillary tube and a water-hydrophilic/hydrophobic organic solvent mixture as a carrier solution ; the system works under laminar flow conditions. The capillary chromatography is called a tube radial distribution chromatography (TRDC) system.

利用层流条件下微通道内液-液界面处的化学发光反应空间，建立了一种新的化学发光分析方法。我们称之为“微通道化学发光分析（MCCLA）”的分析系统。首先，微通道化学发光分析的概念和它的特点进行了描述和考虑，通过使用荧光显微镜CCD相机和显微镜CCD相机的荧光和化学发光的直接观察。接着介绍了几个具体的微通道化学发光分析实例，并对实验数据进行了初步的讨论。通过使用草酸盐化学发光反应的微通道化学发光分析来检测荧光化合物。抗氧，饮料，和唾液也进行了研究，通过化学发光分析，使用单线态氧化学发光反应。此外，我们已经开发了一种毛细管色谱法，使用一个开放的毛细管和水-亲水性/疏水性有机溶剂混合物作为载体溶液;该系统的工作条件下层流。毛细管色谱被称为管径向分布色谱（TRDC）系统。

项目成果

Capillary Chromatography Based on Tube Radial Distribution of Aqueous-Organic Mixture Carrier Solvents : Effect of the Inner Wall Characteristics of the Fused-Silica Tube on Separation Performance

基于水-有机混合物载体溶剂管径向分布的毛细管色谱法：熔融石英管内壁特性对分离性能的影响

• 发表时间: 2009
• 期刊: Analytical Science 25
• 作者: Naoya Jinno;Ko Hashimoto;Masahiko Hashimoto;Kazuhiko Tsukagoshi
• 通讯作者: Kazuhiko Tsukagoshi

Micro-Channel Chemiluminescence Analysis Using Peroxyoxalate Reaction that Works through Liquid Liquid Interface Collapse under Laminar Flow Conditions

利用层流条件下液液界面塌陷的过氧草酸反应进行微通道化学发光分析

• 发表时间: 2008
• 期刊: Analytical Sciences 24
• 作者: Kazuhiko Tsukagoshi;Yoshiyuki Hattori;Teruki Hayashi;Riichiro Nakajima;Kenichi Yamashita;Hideaki Maeda
• 通讯作者: Hideaki Maeda

管径方向分配クロマトグラフィーの開発

径向分配色谱的发展

• 发表时间: 2010
• 作者: 大胡恵樹;根矢三郎;高橋正;橋爪大輔;中村幹夫;塚越一彦;宮崎晃平;Hiroyuki Suga;塚越一彦
• 通讯作者: 塚越一彦

Analysis of antioxidants by microchip capillary electrophoresis with chemiluminescence detection based on luminol reaction

• DOI: 10.1016/j.talanta.2008.03.026
• 发表时间: 2008-12
• 期刊: Talanta
• 影响因子: 6.1
• 作者: K. Tsukagoshi;Takahiro Saito;R. Nakajima

管径方向分配クロマトグラフィー(TRDC)の開発

径向分配色谱（TRDC）的开发

• 发表时间: 2009
• 作者: 大胡恵樹;池崎章;中村幹夫;菅博幸;塚越一彦
• 通讯作者: 塚越一彦