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Development of Whole Cell-Immobilized Biosensors Using Electron Transfer Mediator for Detection of Pollutants in Environment

利用电子转移介体开发全细胞固定化生物传感器来检测环境中的污染物

基本信息

批准号：
11660331
负责人：
MIKI Kojiro
金额：
$ 1.47万
依托单位：
Nara National College of Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11660331/
关键词：
Bioelectrocatalysis Biosensor Whole Cell Electron Transfer Mediator Cyanide Herbicide

项目摘要

Whole cell modified electrode using Baker's yeast cells produced an anodic current approaching a steady state with the addition of electron transfer mediator. When glucose was further added to the solution, the anodic current started to increase again. The current magnitude depends on the concentration of NADH in the cells. The anodic current with glucose increased when yeast cells at logarithmic growth phase were used. The current increased with the addition of KCN due to an increase in the concentration of intracellular NADH.Thus, the electrode employing whole-cells of baker's yeast may be used for the determination of number of cells and the detection of cytotoxic compoundsAn electrode with immobilized mycelium of Aspergillus niger also produced an anodic current with the addition of mediators. The scnsitivity of the current response with carbohydrates was influenced by the cultivation medium in which A.niger was cultured.An electrode with immobilized Chorella vulgaris Beijerinck cell also produced an anodic current with a white light illumination in the presence of p-benzoquinone in a solution. The anodic current is due to the photosynthesis of C.vulgaris. Since a herbicide such as Diuron inhibits the photosynthesis of C.vulgaris, it may be detected by the decrease in the currentGlycolytic oscillation of a metabolite has been successfully monitored with a bioelectrochemical sensor for pyruvate, and the significance of the NAD^+/NADH ratio in the glycolytic oscillation is proposed.

利用贝克酵母细胞修饰的全细胞电极，在加入电子传递介质后产生接近稳态的阳极电流。当葡萄糖进一步加入溶液时，阳极电流又开始增加。电流的大小取决于细胞内NADH的浓度。当酵母细胞处于对数生长期时，葡萄糖的阳极电流增加。随着KCN的加入，由于细胞内NADH浓度的增加，电流增加。因此，采用面包酵母全细胞的电极可用于细胞数量的测定和细胞毒性化合物的检测。采用固定化黑曲霉菌丝体的电极在添加介质后也能产生阳极电流。目前对碳水化合物反应的敏感性受培养黑曲霉的培养基的影响。在对苯醌存在的溶液中，固定化小藻贝耶林克细胞电极也产生白光照明的阳极电流。阳极电流是由紫花草光合作用产生的。由于Diuron等除草剂能抑制c.o argaris的光合作用，因此可以通过降低代谢产物的电流来检测，并利用丙酮酸生物电化学传感器成功地监测了糖酵解振荡，并提出了NAD^+/NADH比值在糖酵解振荡中的意义。