Development of low-cost self-driven coulometric system as science teaching materials and its application to education of science and environment

低成本自驱动库仑系统科学教材的研制及其在科学与环境教育中的应用

• 批准号: 23501046
• 负责人: MIKI Kojiro
• 金额: $ 3万
• 依托单位: Nara National College of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23501046/
• 关键词: クーロメトリー; 自己駆動; 酵素反応; 酸化還元反応; 科学教育; 金属ナノ粒子; 自己駆動型電量分析; 酵素; 電子受容体; 微生物; 化学教育; バイオ電池; 電子伝達メディエータ; 酵母

A new method for determination of D-glucose was developed by combining glucose dehydrogenase (GDH) reaction and a low-cost self-driven coulometric system. Carbon felt was used an electrode material for both the anode and cathode of the coulometric cell. Glucose was oxidized in the presence of GDH and electron acceptor (Ubiquinone 0, UQ) was reduced. To determine the amount of reduced UQ, reduced UQ was oxidized at the anode driven by the electromotive force, and ferricyanide ion containing in the anode solution was reduced. The measured total charge was almost same of the theoretical values obtained from the glucose concentration. Glucose concentrations in sake moromi measured by this method were correlated with those determined by spectrophotometry. Pyruvate could be measured by the system using lactate dehydrogenase, NADH, diaphorase, and UQ.The system could be used as science teaching materials in the chemical experiment of College of Technology and senior high school.

将葡萄糖脱氢酶反应与低成本的自驱式库仑反应相结合，建立了测定D-葡萄糖的新方法。碳毡被用作库仑电池的阳极和阴极。葡萄糖在GDH存在下被氧化，电子受体(Ubiquone 0，UQ)被还原。为了确定还原的UQ的量，在电动势的驱动下，在阳极上氧化还原的UQ，并还原阳极液中的铁氰化钾离子。测得的总电荷与由葡萄糖浓度得到的理论值基本相同。用该方法测定的清酒中的葡萄糖浓度与用分光光度法测定的葡萄糖浓度进行了相关分析。该系统可利用乳酸脱氢酶、NADH、黄递酶、UQ等多种酶测定丙酮酸的含量，可作为理工科大学和高中化学实验的科学教材。

项目成果

逆ミセル有機相への可溶化に伴う金ナノ粒子の分散挙動

金纳米颗粒在反胶束有机相中增溶后的分散行为

• 发表时间: 2012
• 作者: 河田晋治;西野悟;直江一光
• 通讯作者: 直江一光

Development of Biofuel Cell using Baker' s Yeast as Biocatalyst and Ethanol as Fue

以面包酵母为生物催化剂、乙醇为燃料的生物燃料电池的研制

• 发表时间: 2011
• 作者: Kojiro Miki;Ayumu Funabiki;Kenji Kano
• 通讯作者: Kenji Kano

FAD 依存性グルコース脱水素酵素を用いた自己駆動型バイオクーロメトリーによる日本酒モロミ中のグルコース定量

使用 FAD 依赖性葡萄糖脱氢酶通过自驱动生物库仑法测定清酒醪中的葡萄糖

• 发表时间: 2013
• 作者: 川嶋浩平;三木功次郎
• 通讯作者: 三木功次郎

酵母を用いたバイオ電池教材の開発

利用酵母开发生物电池教材

• 发表时间: 2013
• 作者: 三木功次郎;川嶋浩平
• 通讯作者: 川嶋浩平

水溶性金ナノ粒子の調製とそれらが及ぼす微生物細胞への影響

水溶性金纳米颗粒的制备及其对微生物细胞的影响

• 发表时间: 2013
• 作者: 羽谷航;柳瀬剛士;直江一光;澤井淳;今井正直
• 通讯作者: 今井正直