Development of low-cost self-driven coulometric system as science teaching materials and its application to education of science and environment

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A new method for determination of D-glucose was developed by combining glucose dehydrogenase (GDH) reaction and a low-cost self-driven coulometric system. Carbon felt was used an electrode material for both the anode and cathode of the coulometric cell. Glucose was oxidized in the presence of GDH and electron acceptor (Ubiquinone 0, UQ) was reduced. To determine the amount of reduced UQ, reduced UQ was oxidized at the anode driven by the electromotive force, and ferricyanide ion containing in the anode solution was reduced. The measured total charge was almost same of the theoretical values obtained from the glucose concentration. Glucose concentrations in sake moromi measured by this method were correlated with those determined by spectrophotometry. Pyruvate could be measured by the system using lactate dehydrogenase, NADH, diaphorase, and UQ.The system could be used as science teaching materials in the chemical experiment of College of Technology and senior high school.