Development of biofuel cell as a science teaching material and its application to education of environment, energy and biotechnology

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We developed a mediated electron-transfer type biofuel cell using baker's yeast for bread fermentation as biocatalyst, ethanol as fuel. A phosphate buffer solution (pH 7.0) containing ethanol, 2-methyl-1, 4-naphthoquinone and baker's yeast were used as the anodic solution. K_3[Fe(CN)_6] was used as the cathodic solution. Carbon felt was used an electrode material for both the anode and cathode. The biofuel cell could work as an energy source of a small size motor for 1hr and could be used as a science teaching material in the chemical experiment of Junior high school and senior high school.