SYNERGISTIC EFFECTS OF MURINE CYTOMEGALOVIRUS AND GRAFT-VERSUS-HOST REACTION

用鼠巨细胞病毒注射F1杂交小鼠 （MCMV）或亲本脾细胞（移植物抗宿主反应- GVHR）导致快速和严重的免疫抑制。 选择病毒或亲本细胞的接种， 他们将低于严重的阈值， 免疫抑制 然而，当这两个接种体 结合，观察到严重的免疫抑制。 富内斯， 给F1代小鼠注射能识别 只有I类MHC决定簇不会导致免疫性 镇压 然而，一级承认和 MCMV感染导致严重的免疫抑制。 在诱导GVH之前用MCMV感染宿主小鼠 导致免疫抑制增强，而感染 在诱导GVH之前用MCMV的供体小鼠导致 降低免疫抑制。 MCMV和 GVHR也导致间质性肺炎，而 单独的损伤对肺没有可检测的致病作用。 这些研究允许研究免疫抑制 MCMV感染的可能后果 感染并伴有移植物抗宿主反应 在体内对小鼠进行处理， 在L3 T4+或L3 T4+和Lyt 2 + T细胞中，然后用 MCMV。 在L3 T4耗尽后感染MCMV的小鼠存活， 而在L3 T4+和Lyt 2+耗尽后感染的小鼠 细胞死亡。 这一观察结果可能与确定 T细胞亚群对保护免受CMV感染很重要。

Injection of F1 hybrid mice with either murine cytomegalovirus (MCMV) or parental spleen cells (graft-versus-host reaction - GVHR) results in rapid and severe immunosuppression. Inoculation of either the virus or parental cells were selected so that they would be below the threshold for severe immunosuppression. However, when these two inocula were combined, severe immunosuppression was observed. Futhermore, injection of F1 mice with parental lymphocytes that recognize only class I MHC determinants does not result in immune suppression. However, a combination of class I recognition and MCMV infection results in profound immune suppression. Infection of host mice with MCMV prior to induction of GVH resulted in augmented immune suppression, whereas infection of donor mice with MCMV before induction of GVH resulted in reduced immune suppresion. The combination of MCMV and GVHR also resulted in interstitial pneumonitis, whereas either insult alone had no detectable pathogenic effect on the lungs. These studies permit the investigation of the immunosuppression of MCMV infection and the possibility consequences of CMV infection coupled with a GVHR. Mice were treated in vivo in such a way to render them deficient in L3T4+ or both L3T4+ and Lyt2+ T cells, and then infected with MCMV. Mice infected with MCMV after L3T4 depletion survived, whereas mice infected after depletion of both L3T4+ and Lyt2+ cells died. This observation may be relevant for determining the T cell subsets important for protection against CMV infection.