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The Role of age on induction and continuity of autoimmune thyroiditis

年龄对自身免疫性甲状腺炎的诱发和持续性的作用

基本信息

批准号：
63570153
负责人：
OKAYASU Isao
金额：
$ 1.41万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1989
项目状态：
已结题

项目摘要

After induction of experimental autoimmune thyroiditis (EAT) in mice, long-term observation on both the severity of thyroiditis and antibody formation against mouse thyroglobulin (MTg) was carried out over a period of 18 months. Both the severity of thyroiditis and MTg antibody production continued for a long term in EAT-susceptible strains (CBA, C3H). In EAT-resistant mice (BALB/c), thyroiditis remained minimal, if present, and the low antibody response to MTg became negligible. Antibody against sheep red blood cells (SRBC) in serum, also continued for a long term, depending on mouse strains. On the other hand, aging had a prominent effect on the EAT induction in susceptible mice; both MTg antibody response and thyroid infiltration were markedly reduced. These data were well correlated with reduced antibody formation against SRBC. Young and old mice were further compared as donors or recipients in adoptive transfer of concanavalin A-stimulated cells. Lymph node cells from either young or old MTg-immunized mice transferred appreciable thyroiditis to normal young and old recipient mice. However, similarly treated spleen cells from MTg-immunized old mice responded more poorly to in vitro stimulation with mitogens or MTg and transferred thyroiditis to very few normal young or old mice.In next experiment, cloning of effector T cells against thyroid epithelial cells should be performed. Further, the response of in vivo immune system should be studied after injection of cloned effector T cells.

在诱发小鼠实验性自身免疫性甲状腺炎（EAT）后，对甲状腺炎的严重程度和抗小鼠甲状腺球蛋白（MTg）抗体的形成进行了为期18个月的长期观察。甲状腺炎的严重程度和MTg抗体的产生在EAT敏感株（CBA，C3 H）中持续了很长一段时间。在EAT耐药小鼠（BALB/c）中，甲状腺炎（如果存在）仍然很轻微，对MTg的低抗体应答变得可以忽略不计。血清中抗绵羊红细胞（SRBC）的抗体也持续很长时间，这取决于小鼠品系。另一方面，衰老对易感小鼠的EAT诱导有显著影响; MTg抗体反应和甲状腺浸润均显著减少。这些数据与针对SRBC的抗体形成减少密切相关。进一步比较了年轻和老年小鼠作为供体或受体在过继转移刀豆球蛋白A刺激的细胞。来自年轻或年老MTg免疫小鼠的淋巴结细胞将明显的甲状腺炎转移到正常年轻和年老受体小鼠。然而，类似的处理从MTg免疫的老年小鼠的脾细胞在体外刺激有丝分裂原或MTg的反应更差，并转移甲状腺炎，以很少的正常年轻或老年小鼠。此外，应研究在注射克隆的效应T细胞后体内免疫系统的应答。