The role of telomerase activity in thyroid tumorigenesis

端粒酶活性在甲状腺肿瘤发生中的作用

• 批准号: 09670202
• 负责人: OKAYASU Isao
• 金额: $ 1.98万
• 依托单位: KITASATO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670202/
• 关键词: thyroiditis; thyroid tumors; telemerase; telemere length; differentiation; cell proliferation; apoptosis; テロメレーズ

Telomerase activity was examined in thyroid disease states by telomeric repeat amplification protocol assay, including adenomas arid carcinomas, and correlated with Clinicopathological features. Of a total of 26 papillary carcinomas, 16 cases (61.5%) were positive, with the poorly differentiated subtype being predominant (P<O.O5). A significantly more shortened terminal restriction fragment length (P<O.05), higher incidence of extrathyroidal extension (P<O.OO1), and more elevated Ki-67 labeling indices (P<O.002) were also found in telomerase-positive than in -negative papillary carcnnomas. Of four follicular carcinomas, 3 cases (75.5%) were positive. Positive telomerase activity in follicular adenomas (9/23 cases, 39.1%) and lymphocytic thyroiditis (12/22 cases, 54.5%) appeared to be mainly caused by infiltrating lymphocytes. However, three cases of atypical adenoma with relatively increased Ki-67 labeling indices were positive, suggesting a possibility of malignant potential. The good correlation with extrathyroidal invasiveness, Ki-67 labeling indices and poor differentiation of papillary carcinomas which was determined by the use of multivariate analysis suggests that this parameter might have potential application in estimation of tumor progession and prognosis and in clinical management.Similarly, the relation of telomerase activity with cancer cell differentiation, proliferation and progression was confirmed in sporadic colorectal carcinomas.

通过端粒重复扩增方案检测甲状腺疾病状态（包括腺瘤和癌）的端粒酶活性，并与临床病理学特征相关。 26例乳头状癌中，16例（61.5%）呈阳性，以低分化亚型为主（P<0.05）。端粒酶阳性的乳头状癌与端粒酶阴性的乳头状癌相比，末端限制性片段长度显着缩短（P<O.05），甲状腺外延伸的发生率更高（P<O.001），Ki-67标记指数更高（P<O.002）。 4 例滤泡性癌中，3 例（75.5%）呈阳性。滤泡性腺瘤（9/23例，39.1%）和淋巴细胞性甲状腺炎（12/22例，54.5%）端粒酶活性阳性似乎主要是由浸润淋巴细胞引起的。然而，3例Ki-67标记指数相对升高的非典型腺瘤呈阳性，提示有恶变的可能性。通过多变量分析确定，该参数与甲状腺外侵袭性、Ki-67标记指数和乳头状癌分化差具有良好的相关性，表明该参数在评估肿瘤进展和预后以及临床治疗中可能具有潜在的应用价值。同样，在散发性结直肠癌中端粒酶活性与癌细胞分化、增殖和进展的关系也得到证实。 癌。

项目成果

Okayasu,I.: "The relationship of lymphocytic thyroiditis to the development of thyroid carcinoma" Endocrine pathology. 8・3. 225-230 (1997)

冈安，I.：“淋巴细胞性甲状腺炎与甲状腺癌发展的关系”内分泌病理学8・3（1997）。

Okayasu I,Osakabe T,Fujiwara M,Fukuda H,Kato M,Oshimura M.: "Significant correlation of telomerase activity in thyroid papillary carcinomas with cell differentiation, proliferation and extrathyroidal extension" Jpn J Cancer Res. 88. 965-970 (1997)

Okasu I、Osakabe T、Fujiwara M、Fukuda H、Kato M、Oshimura M.：“甲状腺乳头状癌中端粒酶活性与细胞分化、增殖和甲状腺外延伸的显着相关性”Jpn J Cancer Res。

Okayasu,I.et al: "Telorase activity significantly correlates with cell differentiation proliferation and lymph node metastasis in colorectal carcinomas" J Cancer Res Clin Onco1. 124. 444-449 (1998)

Okayasu,I.et al：“Telorase 活性与结直肠癌中的细胞分化增殖和淋巴结转移显着相关”J Cancer Res Clin Onco1。

Okayasu I, et al: "p53 and p21 WAF1 expression in lymphocyticthyroiditis and thyroid tumors." C1in Immuno1 Immunopathol. 88. 183-191 (1998)

Okayasu I 等人：“p53 和 p21 WAF1 在淋巴细胞性甲状腺炎和甲状腺肿瘤中的表达。”

Okayasu I,Mitomi H,Yamashita K,Mikami T,Fujiwara M,Kato M,Oshimura M: "Telomerase activity signfificantly correlates with cell differentiation, proliferation and lymph node metastasis in colorectal carcinomas." J Cancer Res Clin Oncol. 124. 444-449 (1998)

Okasu I、Mitomi H、Yamashita K、Mikami T、Fujiwara M、Kato M、Oshimura M：“端粒酶活性与结直肠癌的细胞分化、增殖和淋巴结转移显着相关。”