Regulation of cytokine production through the receptors for lysophospholipid in mast cells

通过肥大细胞中溶血磷脂受体调节细胞因子的产生

基本信息

  • 批准号:
    11670434
  • 负责人:
  • 金额:
    $ 2.3万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    1999
  • 资助国家:
    日本
  • 起止时间:
    1999 至 2000
  • 项目状态:
    已结题

项目摘要

We found that mast cells express receptors for lysophspholipid. However, we cannot find the evidence that lysophospholipid modulates IgE Fc receptor (FcεRI)-mediated cytokine production in mast cells. We expects one of protein kinase C (PKC) members, PKCμ, is involved in FcεRI-mediated C-Jun amino-terminal kinase (JNK) activation and cytokine production in mast cells because only PKC inhibitors which inhibit PKCμ abolish FcεRI-mediated JNK activation but not those which cannot inhibit PKCμ. Although an accumulation of mast cells within the epithelial layer of the mucosa is observed in patients with allergic rhinitis and bronchial asthma, the responsible chemotactic factors are undefined. We investigated whether mast cells sensitized with antigen-specific IgE migrate toward the antigen or not. MC/9 mast cells sensitized with anti-dinitrophenyl (DNP) IgE migrated toward DNP conjugated human serum albumin (DNP-HSA). This migration was directional, and the degree of migration was stronger … More than that of stem cell factor (SCF)-induced migration. Mouse bone marrow-derived cultured mast cells (BMMC) also migrated toward the antigen. The high-affinity IgE Fc receptor (FcεRI)-mediated migration was significantly inhibited by pretreatment of-the cells with Y-27632, a Rho-associated coiled-coil forming protein kinase (ROCK) inhibitor, or SB 203580, a p38 MAP kinase (p38 MAPK) inhibitor. Both p38 MAPK and MAPKAP kinase 2 were activated following aggregation of FcεRI, and activation of MAPKAP kinase 2 was almost completely inhibited by 10μM SB203580. Partial inhibition of MAPKAP kinase 2 by pretreatment with wortmannin or a low concentration of SB203580 was not enough to block mast cell migration. On the other hand, Y-27632 did not affect the activation of MAPKAP kinase 2. These results indicate that antigen works not only as a stimulant for allergic mediators from IgE-sensitized mast cells but also as a chemotactic factor for mast cells. Both p38 MAPK activation and Rho-dependent activation of ROCK may be required for FcεRI-mediated cell migration. Less
我们发现肥大细胞表达溶血磷脂受体。然而,我们没有发现溶血磷脂调节肥大细胞Ig E Fc受体(FcεRI)介导的细胞因子产生的证据。我们认为蛋白激酶C成员之一PKCμ参与了FcεRI介导的肥大细胞C-jun氨基末端激酶的激活和细胞因子的产生,因为只有抑制PKCμ的PKC抑制剂才能取消FcεRI介导的JNK激活,而不能抑制PKCμ的激活。虽然在变应性鼻炎和哮喘患者中观察到粘膜上皮层中肥大细胞的聚集,但其相关的趋化因素尚不明确。我们研究了用抗原特异性IgE致敏的肥大细胞是否向抗原迁移。抗二硝基苯基(DNP)IgE致敏的MC/9肥大细胞向DNP结合的人血清白蛋白(DNP-HSA)迁移。这种迁移是定向的,而且迁移的程度更强…比干细胞因子(SCF)诱导的迁移更多。小鼠骨髓来源的肥大细胞(BMMC)也向该抗原迁移。Rho相关卷曲形成蛋白激酶(ROCK)抑制剂Y-27632或p38 MAPK(P38MAPK)抑制剂SB 203580可显著抑制高亲和力IgEFc受体(FcεRI)介导的迁移。P38MAPK和MAPKAPK2在FcεRI聚集后均被激活,10μM SB203580几乎完全抑制MAPKAPK2的激活。Wortmannin或低浓度SB203580对MAPKAP激酶2的部分抑制不足以阻断肥大细胞的迁移。另一方面,Y-27632不影响MAPKAP-K2的激活。这些结果表明,该抗原不仅作为Ig E致敏的肥大细胞变态反应介质的刺激剂,而且还作为肥大细胞的趋化因子。FcεRI介导的细胞迁移可能需要p38MAPK激活和Rho依赖的ROCK激活。较少

项目成果

期刊论文数量(2)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
石塚全 ほか: "Cyclospovin A (CsA)は肥満細胞の抗原刺激によるc-Jun amino-terminal kinase(JNK)活性化を抑制する"呼吸. 19. 61-62 (2000)
Zen Ishizuka 等人:“环孢素 A (CsA) 抑制肥大细胞抗原刺激诱导的 c-Jun 氨基末端激酶 (JNK) 激活”呼吸研究 19. 61-62 (2000)。
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石塚全 ほか: "Cyclosporin A (CsA)は肥満細胞の抗原刺激によるc-Jun amino-terminal kinase (JNK)活性化を抑制する"呼吸. 19. (2000)
Zen Ishizuka 等人:“环孢素 A (CsA) 抑制肥大细胞抗原刺激诱导的 c-Jun 氨基末端激酶 (JNK) 激活”,呼吸研究 19。(2000)。
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ISHIZUKA Tamotsu其他文献

ISHIZUKA Tamotsu的其他文献

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{{ truncateString('ISHIZUKA Tamotsu', 18)}}的其他基金

Function and intracellular signaling of human umbilical cord blood-derived cultured basophils
人脐带血培养嗜碱性粒细胞的功能和细胞内信号传导
  • 批准号:
    20591183
  • 财政年份:
    2008
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Chronic airway inflammation regulated by proton-sensing receptors
质子敏感受体调节的慢性气道炎症
  • 批准号:
    18591099
  • 财政年份:
    2006
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Sphingosine 1-phosphate inhibits airway wall remodeling in bronchial asthma
1-磷酸鞘氨醇抑制支气管哮喘气道壁重塑
  • 批准号:
    15591045
  • 财政年份:
    2003
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Research on migration of sensitized mast ceils toward the antigen
致敏肥大细胞向抗原迁移的研究
  • 批准号:
    13670445
  • 财政年份:
    2001
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

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