The clarification of pathology of allergic Reactions to gelatin-containing vaccine.

明胶疫苗过敏反应病理学的澄清。

• 批准号: 11670771
• 负责人: TSUTSUMI Hiroyuki
• 金额: $ 2.11万
• 依托单位: Sapporo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670771/
• 关键词: gelatin; gelatin allergy; Th1; Th2; IL-4; IL-13; IL-2; IFN-γ

We investigated the characteristics of T lymphocytes from patients allergic to gelatin-containing vaccine by secondary in vitro stimulation of circulating mononuclear cells with gelatin.Patients with an immediate reaction all had gelatin-specific IgE antibody, whereas others did not. Lymphocytes from subjects with nonimmediate-type reactions generally expressed very weak or sometimes no IFN-γ, IL-2 , or IL-13 genes and especially no IL-4 gene. On the other hand, the lymphocytes of subjects with immediate-type reactions significantly expressed not only IL-4 and IL-13 but also IFN-γ and IL-2 mRNA.These suggest that both gelatin-specific Th2 and Th1 responses are involved in the pathogenesis of the immediate reaction to gelatin. The gelatin-specific IL-4 and/or IL-13 responses consistently observed in patients with an immediate reaction may be associated with the production of gelatin-specific IgE antibody.

我们用明胶体外二次刺激循环单个核细胞，研究了对明胶疫苗过敏的患者的T淋巴细胞特性，立即反应的患者均具有明胶特异性IgE抗体，而其他患者则没有。非速发型反应受试者的淋巴细胞通常表达非常弱或有时不表达IFN-γ、IL-2或IL-13基因，尤其是不表达IL-4基因。另一方面，明胶即刻反应患者的淋巴细胞不仅表达IL-4和IL-13，而且还表达IFN-γ和IL-2 mRNA，提示明胶特异性Th 2和Th 1应答参与了明胶即刻反应的发病机制。在即刻反应患者中持续观察到的明胶特异性IL-4和/或IL-13应答可能与明胶特异性IgE抗体的产生相关。

项目成果

Kumagai T, Nakayama T, Kamada M, Igarashi C, Yuri K, Furukawa H, Wagatsuma K, Tsutsumi H, Chiba S, Kojima H, Saito A, Okui T, Yano S: "The lymphoproliferative response to enzymatically digested gelatin in subjects with gelatin hypersensitivity."Clin Exp A

Kumagai T、Nakayama T、Kamada M、Igarashi C、Yuri K、Furukawa H、Wagatsuma K、Ttsutsumi H、Chiba S、Kojima H、Saito A、Okui T、Yano S：“受试者对酶消化明胶的淋巴增殖反应

Kumagai T,Tsutsumi H et al.: "The lymphoprolife rative response to-"Clin Exp Allergy. 30. 1430-1435 (2000)

Kumagai T、Tsutsumi H 等人：“淋巴细胞增殖反应对”Clin Exp 过敏。

Ohsaki M,Tsutsumi H, et al: "The relevance of Th1 and Th2 cells-"J Allergy Cliu Immunol. 103. 276-281 (1999)

Ohsaki M、Tsutsumi H 等人：“Th1 和 Th2 细胞的相关性 -”J Allergy Cliu Immunol。

Ohsaki M, Tsutsumi H, et al: "The relevance of Th1 and Th2 cells in immediate〜"J Allergy Clin Immuocol. 103. 276-281 (1999)

Ohsaki M、Ttsutsumi H 等：“Th1 和 Th2 细胞的相关性〜”J Allergy Clin Immuocol. 103. 276-281 (1999)

Kumagai T, Tsutsumi H, et al: "The lymphoproliferative response to enzymatically"Clin Exp Allergy. (in press).

Kumagai T、Ttsutsumi H 等人：“酶促淋巴增殖反应”Clin Exp Allergy。