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The effect of RSV genotypes on the development of asthma after RSV lower respiratory tract infection

RSV基因型对RSV下呼吸道感染后哮喘发生的影响

基本信息

批准号：
17591101
负责人：
TSUTSUMI Hiroyuki
金额：
$ 1.86万
依托单位：
Sapporo Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591101/
关键词：
Infection Bronchial asthma Allergy 分子疫学 RSウイルス RSウイルスグループA RSウイルスグループB

项目摘要

The circulation pattern and genetic evolution of respiratory syncytial virus (RSV) in Japan were examined based on 109 RSV field strains isolated over 20 seasons (1980-2002) in two cities, Sapporo and Tokyo. The second hypervariable region of the large glycoprotein (G) gene was amplified by RT-PCR and the products sequenced directly. The nucleotide sequences were compared to those representatives of RSV genotypes identified previously. Japanese group A and B isolates clustered into five and four genotypes defined previously, respectively. Another one group A and one group B genotypes, which could not be assigned to previous genotypes, were also identified. Although different genotypes usually co-circulated in each season, the isolates in proximate seasons from two communities were usually located in the same branches. Moreover, the strains with genotypes defined previously were usually isolated at the same time as each reference strain of Western countries. Several mutant group B strains with 1-20 longer amino acid G proteins were newly identified in Sapporo. These findings suggest that Japanese RSV strains underwent geographical and also temporal clustering while participating in RSV genetic evolution in a global setting. In addition, Japanese strains, especially group B, might have evolved individually in each community, sometimes changing the length of the G protein.Next, the relationship between RSV subgroup / genotype and the prognosis of RSV lower respiratory tract infection, that is recurrent wheezing or bronchial asthma were investigated. However, no apparent relationship of particular subgroup / genotype and these diseases.

根据1980-2002年在日本两个城市札幌和东京20个季节分离的109株呼吸道合胞病毒（RSV）野毒株，研究了日本RSV的循环模式和遗传进化。采用RT-PCR方法扩增大糖蛋白（G）基因第二高变区，产物直接测序。将核苷酸序列与先前鉴定的RSV基因型的代表进行比较。日本A组和B组分离株分别聚为5种和4种基因型。另外还鉴定了一个A组和一个B组基因型，这些基因型不能归属于以前的基因型。虽然不同的基因型在不同的季节通常是共同循环的，但来自两个群落的近季节的菌株通常位于同一分支。此外，具有先前定义的基因型的菌株通常与西方国家的每个参考菌株同时分离。在札幌新鉴定了几个G蛋白氨基酸长度延长1-20个的突变型B群菌株。这些结果表明，日本RSV毒株经历了地理和时间聚类，同时参与全球背景下的RSV遗传进化。此外，日本株，特别是B组，可能在每个群体中单独进化，有时改变G蛋白的长度。接下来，研究RSV亚群/基因型与RSV下呼吸道感染的预后（即反复喘息或支气管哮喘）之间的关系。然而，没有明显的特定亚群/基因型与这些疾病的关系。

项目成果

期刊论文数量（15）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

新感染症学感染症学各論 RSウイルス感染症

新传染病、传染病、RS病毒感染

DOI：
发表时间：
2007
期刊：
日本臨床 65
影响因子：
0
作者：
Waguri S;Tomiyama Y;Ikeda H;Hida T;Sakai N;Taniike M;Ebisu S;Uchiyama Y.;堤裕幸
通讯作者：
堤裕幸

Early-onset sarcoidosis and CARD15 mutations with constitutive nuclear factor-κB activation:: common genetic etiology with Blau syndrome

DOI：
10.1182/blood-2004-07-2972
发表时间：
2005-02-01
期刊：
BLOOD
影响因子：
20.3
作者：
Kanazawa, N;Okafuji, I;Miyachi, Y
通讯作者：
Miyachi, Y

Early-onset sarcoidosis and CARD15 mutations with constirutive nuclear factor-κB activation : common gentic etiology with Blau syndrome.

早发性结节病和 CARD15 突变伴组成型核因子-κB 激活：Blau 综合征的常见遗传病因。

DOI：
发表时间：
2005
期刊：
Blood Vol 105/3
影响因子：
0
作者：
Tsujita Y;Muraski J;Shiraishi I;Kato T;Kajstura J;Anversa P;Sussman MA;Nobuo kanazawa et al.
通讯作者：
Nobuo kanazawa et al.

A phylogenetic study of human respiratory syncytial viruses group A and B strains isolated in two cities in Japan from 1980-2002