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Analysis of pathogenesis of primary infection and reactivation of HHV-6

HHV-6原发感染及再激活发病机制分析

基本信息

批准号：
11670802
负责人：
YOSHIKAWA Tetsushi
金额：
$ 1.09万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670802/
关键词：
HHV-6 primary infection reactivation cell proliferation 非RI BrdU

项目摘要

1) Lymphoproliferative response against HHV-6 in patients with exanthem subitum : Kinetics of the lymphoproliferative response (stimulation index (S.I.)), which was evaluated by Cell Proliferation ELISA (Boehringer), after primary HHV-6 infection is as follows ; acute phase : 1.2, 1 week after infection : 1.3, 1month after infection : 1.9. All S.I. Were lower than that in healthy adults (S.I.=5. 1).2) Immunosuppression after primary HHV-6 infection : In order to evaluate immunological status hi each patient, lymphoproliferative response by PHA stimulation was tested hi all samples. Lymphoproliferative response by the stimulation decreased clearly during first 1 -month period after the virus infection. This result suggests that non-specific immune response could be impaired during 1 month after primary HHV-6 infection.3) Purification of the virus antigeif In order to improve non-specific stimulation by the crude antigen, the antigen was purified by density gradient method. Although the purified antigen demonstrated lower background value in most samples, some samples still showed high background value. It is speculated that HLA type of cord blood mononuclear cells that is used for preparation of the antigen, could cause the problem.4) HHV-6 infection hi K562 cells : Since K562 cells dose not express HLA class I antigen on the surfaces, we attempted to infect HHV-6 into the cells. However, efficiency of the virus infection hi the cells was very low.

1)皮疹患者对HHV-6的淋巴增殖反应：淋巴增殖反应的动力学（刺激指数（S.I.）），通过细胞增殖ELISA（Boehringer）评估，在原发性HHV-6感染后，如下：急性期：1.2，感染后1周：1.3，感染后1个月：1.9。所有SI低于健康成人（S.I.= 5. 1）. 2）原发性HHV-6感染后的免疫抑制：为了评估每个患者的免疫状态，在所有样品中测试通过PHA刺激的淋巴增殖反应。在病毒感染后的前1个月内，由刺激引起的细胞增殖反应明显下降。结果表明，HHV-6感染后1个月内，机体非特异性免疫反应减弱。3）病毒抗原的纯化为了提高抗原的非特异性刺激性，采用密度梯度法纯化抗原。虽然纯化抗原在大多数样品中显示出较低的背景值，但部分样品仍显示出较高的背景值。4）HHV-6感染K562细胞：由于K562细胞表面不表达HLA-I类抗原，因此我们尝试将HHV-6感染到K562细胞中。然而，病毒在细胞中的感染效率非常低。