Basic research for the development of electroporation-electrode-mounted balloon catheter

电穿孔电极球囊导管研制的基础研究

• 批准号: 11670888
• 负责人: YAMADA Kazunari
• 金额: $ 2.37万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670888/
• 关键词: electroporation; gene transfer; imaging guidance; gene therapy; 遺伝子導入; CT-guidance; 遺伝子治療

Purpose : To develop the effective way to transfer genes to the vessel wall by using the electroporation Materails and methods : 1 : We developed the percutaneously insertable electroporation needle, and performed the reporter gene transfer to the thigh muscles of the rabbits. Basic structure of the percutaneously insertable electroporation needle is composed of two outer needles separated by a distance of 10 mm and the DNA injection needle placed in the halfway of two outer needles. Two outer needles and DNA injection needle can be simultaneously inserted. After the retraction of the outer needles, electroporation needles can be exposed to the target area. Without imaging guidance, two outer needles the DMA needle were inserted to the thigh muscles. Then, after the green fluorescent protein (GFP) was injected, electroporation was performed. Three days later, gene transfer was checked.2 : Under imaging guidance, the electroporation needles and were inserted to the thigh muscle of the r … More abbits. Under CT-guidance, two outer needles the DNA needle were inserted to the thigh muscles Then, after the green fluorescent protein (GFP) was injected, electroporation was performed. Three days later, gene transfer was checked.3 : Percutaneously insertable electroporation balloon catheter models were designed, and evaluated its feasibility for the in vivo use. Also, the balloon catheter was inserted to the femoral artery and renal artery of the rabbits under imaging guidance to evaluate the visibility and the effect to the vessel wall.ResultsBoth with and without imaging guidance, the gene expression was confirmed in the area of the thigh between the two electroporaton needles. Without imaging guidance, it was not possible to detect the precise position of the needle tip.Two types of the electroporation balloon catheter were designed. A) Electroporation wires were attached to the balloon in a parallel way. Wires are compressed to the vessel wall when the balloon is inflated.B) Electroporation needles were expanded from the balloon catheter vertically to the vessel wall. However, at the present time, these designs are difficult to be applied in vivo, and needed the further improvement and evaluation. Less

目的：利用电穿孔材料和方法，探索将基因导入血管壁的有效途径：1.研制了可插入式电穿孔针，并将报告基因导入家兔大腿肌肉。永久插入式电穿孔针的基本结构由两个相距10 mm的外针和置于两个外针中间的DNA注射针组成。可同时插入两个外针和DNA注射针。在外部针缩回之后，电穿孔针可以暴露于目标区域。在没有成像引导的情况下，将DMA针的两个外针插入大腿肌肉。然后，在注入绿色荧光蛋白（GFP）后，进行电穿孔。3天后，检查基因转移。2：在成像引导下，将电穿孔针插入受试者的大腿肌肉。 ...更多信息 兔子在CT引导下，将DNA针的两个外针插入大腿肌肉，然后，在注射绿色荧光蛋白（GFP）后，进行电穿孔。3、设计经皮穿刺电穿孔球囊导管模型，评价其在体内应用的可行性。此外，将球囊导管插入到股动脉和肾动脉的兔在成像引导下，以评估的能见度和效果的vessel wall.ResultsBoth有和没有成像引导，基因表达被证实在该地区的大腿之间的两个电穿孔针。由于没有影像学的引导，无法精确的检测针尖的位置，因此设计了两种类型的电穿孔球囊导管。A）将电穿孔丝以平行方式附接到球囊。当球囊膨胀时，金属丝被压缩到血管壁。B）电穿孔针从球囊导管垂直地扩展到血管壁。然而，目前这些设计还难以在体内应用，需要进一步的改进和评价。少