Tandem mass spectrometer coupled to an ultra-high performance liquid chromatograph: UHPLC-HRMS/MS

随着目前为UHPLC-HRMS/MS系统申请大型设备资金，生物过程工程研究所(BVT)正在追求进一步发展的基本目标：(1)巩固酶催化领域的成功项目，(2)使用具有生物活性的天然物质，以及(3)发展微生物生物技术和动物细胞培养领域的系统生物学项目。这里描述的设备对于两位新任命的教授Kathrin Castiglione(生物过程工程)和Sebastian Aljoscha Wahl(系统生物技术)2018年和2022年的计划工作至关重要。该设备将能够在小分子领域进行精确的分析，以识别和量化反应混合物、天然物质、介质以及细胞中的物质(代谢组学)。此外，该设备将使蛋白质组成的测量成为可能。只有使用高分辨率、高灵敏度的系统才能测量复杂样品中的这种复杂分子。该采购由该大学以预约承诺的形式提供财政支持。计划研究的目的是开发和应用工具，用于：(1)研究和优化复杂的生物催化过程，(2)鉴定生物活性天然物质，以开发针对病毒和细菌的天然防御策略，以及(3)(动物)细胞的系统生物学分析。该研究所提供的复杂催化剂、生物过程、培养、新陈代谢和建模方面的知识，为利用HR-MS/MS解决新的科学问题提供了一个很好的起点。

With the present application for large-scale equipment funding for a UHPLC-HRMS/MS system, the Institute of Bioprocess Engineering (BVT) is pursuing essential goals for further development: (1) The consolidation of successful projects in the field of enzymatic catalysis and (2) the use of natural substances with biological activity, as well as (3) the development of systems biology projects in the field of microbial biotechnology and animal cell culture. The device described here is essential for the planned work of the two newly appointed professors Kathrin Castiglione (Bioprocess Engineering) and Sebastian Aljoscha Wahl (Systems Biotechnology) in 2018 and 2022. The device will enable precise analyses in the field of small molecules for the identification and quantification of substances in reaction mixtures, natural substances, media as well as in cells (metabolomics). Furthermore, the device will enable the measurement of protein composition. Measurements of such complex molecules in complex samples can only be achieved with a high-resolution, sensitive system. The procurement is financially supported by the university in the form of appointment commitments. The aim of planned research is to develop and apply tools for: (1) the investigation and optimisation of complex biocatalytic processes, (2) the identification of bioactive natural substances for the development of natural defense strategies against viruses and bacteria, and (3) the systems biology analysis of (animal) cells. The knowledge of complex catalyses, bioprocesses, cultivation, metabolism and modelling available at the institute offers a very good starting point for addressing new scientific questions with the help of HR-MS/MS.