Molecular Cloning of New Sodium Chloride Transporters

新型氯化钠转运蛋白的分子克隆

• 批准号: 11671044
• 负责人: ISHIBASHI Kenichi
• 金额: $ 1.34万
• 依托单位: Jichi Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671044/
• 关键词: TRANSPORTER; ION CHANNEL; MEMBRANE PROTEIN; CLONING; ELECTROLYTE; GLUCOSE; GENE FAMILY; XENOPUS OOCYTE; 輸送体; ナトリウム; カリウム; クロライド; リン酸; 硫酸; カルシウム; 分子生物学; イオン輸送体; クロライド輸送体; 膜たんぱく

The aim of this project was to identify transporters and channels that transport sodium and/or chloride as many as possible so that we can get the clearer view on water and electrolyte metabolism in our bodies. As the transport of water is depend on the osmotic drive by NaCl, water channels (aquaporins) were also searched for. With the advent of genome projects, the process of cloning of new genes was extremely facilitated. Taking advantage of this, several new genes were cloned and the tissue distributions were examined. Some of them were functionally characterized with Xenopus oocyte expression system. Three new Cation-Chloride-Cotransporters (CCC6, CCC7, CCC8), a new sodium-phosphatecotransporter, a new anion exchanger, two new chloride/sulfate exchangers, a novel form of cation channel (Two-Pore-Channel 1 TPC1), 8 new tetraspanning channels, two new capsaicin receptor-like channcls, two new aquaporin-like chloride channels, a new sodium-chloride-catecholamine transporter and a new glucose transporter. Further characterization of thesc membrane proteins are necessary with collaborative works together with experts in the specific fields.

这个项目的目的是确定尽可能多地运输钠和/或氯的转运体和通道，以便我们能够更清楚地了解我们体内的水和电解质代谢。由于水的运输依赖于氯化钠的渗透驱动，因此还寻找了水通道(水通道蛋白)。随着基因组计划的到来，克隆新基因的过程变得极其便利。利用这一点，克隆了几个新的基因，并对其组织分布进行了检测。利用非洲爪哇卵母细胞表达系统对其中的部分进行了功能鉴定。三个新的阳离子-氯离子转运体(CCC6，CCC7，CCC8)，一个新的钠-磷酸转运体，一个新的阴离子交换器，两个新的氯/硫酸盐交换器，一个新的阳离子通道(两孔通道1 TPC1)，8个新的四跨通道，两个新的辣椒素受体类通道，两个新的水通道样氯离子通道，一个新的氯化钠-儿茶酚胺转运体和一个新的葡萄糖转运体。在与特定领域的专家合作的情况下，有必要进一步表征SSC膜蛋白。

项目成果

Suzuki M,Ishibashi K, et al.: "Electrophysiologic characteristics of the Ca-permeable channels, ECaC and CaT, in the kidney."Biochem Biophys Res Commun.. 274(2). 344-349 (2000)

Suzuki M、Ishibashi K 等人：“肾脏中 Ca 渗透通道、ECaC 和 CaT 的电生理特征。”Biochem Biophys Res Commun. 274(2)。

Ohki G,Miyoshi T,Murata M,Ishibashi K, et al.: "A calcium-activated cation current by an alternatively spliced form of Trp3 in the heart"J Biol Chem.. 275(50). 39055-39060 (2000)

Ohki G、Miyoshi T、Murata M、Ishibashi K 等人：“心脏中 Trp3 的选择性剪接形式产生的钙激活阳离子电流”J Biol Chem.. 275(50)。

Kenichi Ishibashi and Masashi Imai.: "Identification of four new members of the rat prolactin/growth hormone gene family."Biochem. Biophys. Res. Commun.. 262. 575-578 (1999)

Kenichi Ishibashi 和 Masashi Imai.：“鉴定大鼠催乳素/生长激素基因家族的四个新成员。”Biochem。

Ishibashi K,Suzuki M,Sasaki S,Imai M.: "Identification of a new multigene four-transmembrane family (MS4A) related to CD20, HTm4 and β subunit of the high-affinity IgE receptor."GENE. (in press). (2001)

Ishibashi K、Suzuki M、Sasaki S、Imai M.：“鉴定与高亲和力 IgE 受体的 CD20、HTm4 和 β 亚基相关的新多基因四跨膜家族 (MS4A)。”GENE。 (2001)

Ohki G, Miyoshi T, Murata M, Ishibashi K, Imai M, Suzuki M: "A calcium-activated cation current by an alternatively spliced form of Trp3 in the heart."J Biol Chem. 2000 Dec15. 275(50). 39055-60

Ohki G、Miyoshi T、Murata M、Ishibashi K、Imai M、Suzuki M：“心脏中 Trp3 的选择性剪接形式产生的钙激活阳离子电流。”J Biol Chem。