Molecular Cloning of New Sodium Chloride Transporters

该项目的目的是确定尽可能多地运输钠和/或氯化物的转运蛋白和通道，以便我们能够更清楚地了解我们体内的水和电解质代谢。由于水的运输依赖于NaCl的渗透驱动，因此也寻找了水通道（水通道蛋白）。随着基因组计划的出现，克隆新基因的过程非常方便。利用这一点，克隆了几个新的基因，并检查了组织分布。其中部分用爪蟾卵母细胞表达系统进行了功能鉴定。3种新型阳离子-氯离子共转运体（CCC6、CCC7、CCC8）、1种新型磷酸钠共转运体、1种新型阴离子交换体、2种新型氯离子/硫酸盐交换体、1种新型阳离子通道（two -孔- channel 1 TPC1）、8种新型四跨通道、2种新型辣椒素受体样通道、2种新型水通道样氯离子通道、1种新型氯化钠-儿茶酚胺转运体和1种新型葡萄糖转运体。进一步表征这些膜蛋白是必要的，与特定领域的专家共同合作。

The aim of this project was to identify transporters and channels that transport sodium and/or chloride as many as possible so that we can get the clearer view on water and electrolyte metabolism in our bodies. As the transport of water is depend on the osmotic drive by NaCl, water channels (aquaporins) were also searched for. With the advent of genome projects, the process of cloning of new genes was extremely facilitated. Taking advantage of this, several new genes were cloned and the tissue distributions were examined. Some of them were functionally characterized with Xenopus oocyte expression system. Three new Cation-Chloride-Cotransporters (CCC6, CCC7, CCC8), a new sodium-phosphatecotransporter, a new anion exchanger, two new chloride/sulfate exchangers, a novel form of cation channel (Two-Pore-Channel 1 TPC1), 8 new tetraspanning channels, two new capsaicin receptor-like channcls, two new aquaporin-like chloride channels, a new sodium-chloride-catecholamine transporter and a new glucose transporter. Further characterization of thesc membrane proteins are necessary with collaborative works together with experts in the specific fields.