The function and the physiological importance of a novel aquaporin family.

新型水通道蛋白家族的功能和生理重要性。

• 批准号: 13671124
• 负责人: ISHIBASHI Kenichi
• 金额: $ 2.11万
• 依托单位: Jichi Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671124/
• 关键词: aquaporin; MIP protein; NPA box; glycerol; testis; pancreas; MDCK細胞; グリセリンチャネル; AQP11; AQP12; グリア細胞; イオンチャネル; 分子生物学; 膵外分泌; アシドーシス; 性成熟; 尿細管

Eleven aquaporins are identified in mammals. They all have highly conserved two asparagine-proline-alanine (NPA) boxes that are important for the formation of water permeating pore. We identified two new proteins in mammals distantly related to aquaporins with poorly conserved NPA repeats. They are named AQP11 and AQP12 (Nomenclature Committee confirmed). Both share a distinct genome structure from known aquaporins. When expressed in Xenopus oocytes, both functioned as glycerol transport facilitators with poor water permeabilities. AQP11 was expressed predominantly in the testis with minor expression in the kidney, liver, and brain. AQP11 expression in the rat testis was developmentally regulated and first appears 25 days after birth, corresponding to the start of meiosis, and is expressed specifically in spermatogonia. AQP12 was expressed selectively in the pancreas, and localized specifically at the basolateral membrane of acinar cells. Its expression was down-regulated by fasting. The identification of a new superfamily of aquaporin will provide new insights into structure-function relationship of aquaporins and deepen our understanding of solute transports in our bodies. Future research will include the knock-out mice of each of these aquaporins and the identification of the diseases associated with these channels.

在哺乳动物中发现了11种水通道蛋白。它们都有两个高度保守的天冬酰胺-脯氨酸-丙氨酸(NPA)盒，这两个盒对透水孔的形成非常重要。我们在哺乳动物中发现了两种新的蛋白质，它们与水通道蛋白有较远的亲缘关系，但NPA重复序列保守程度较低。它们被命名为AQP11和AQP12(命名委员会确认)。两者都与已知的水通道蛋白有着截然不同的基因组结构。当在非洲爪哇卵母细胞中表达时，两者都作为甘油运输促进剂发挥作用，但透水性较差。AQP11主要在睾丸中表达，肾脏、肝脏和脑中也有少量表达。AQP11在大鼠睾丸中的表达受发育调控，在出生后25d开始出现，对应于减数分裂的开始，并在精原细胞中特异表达。AQP12选择性表达于胰腺，定位于腺泡细胞的基底膜。禁食使其表达下调。一个新的水通道蛋白超家族的发现将对水通道蛋白的结构-功能关系提供新的见解，并加深我们对体内溶质运输的理解。未来的研究将包括每一种水通道蛋白的敲除小鼠，以及与这些通道相关的疾病的识别。

项目成果

Morinaga T, Ishibashi K et al.: "Mouse aquaporin 10 gene (AQP 10) is a pseudogene"Biochem Biophys Res Commun. 294. 630-634 (2002)

Morinaga T、Ishibashi K 等人：“小鼠水通道蛋白 10 基因 (AQP 10) 是一个假基因”Biochem Biophys Res Commun。

Ishibashi K, Makoto Suzuki, Sei Sasaki, Masashi Imai: "Identification of a new multigene four-transmembrane family (MS4A)related to CD20, HTm4 and b subunit of the high-affinity IgE receptor"GENE. 264. 87-93 (2001)

Ishibashi K、Makoto Suzuki、Sei Sasaki、Masashi Imai：“鉴定与高亲和力 IgE 受体的 CD20、HTm4 和 b 亚基相关的新多基因四跨膜家族 (MS4A)”GENE。

Suzuki M, Ohki G, Ishibashi K, Imai M: "A single amino acid mutation results in a rapid inactivation of epithelial calcium channels"Biochem Biophys Res Commun. 291. 278-285 (2002)

Suzuki M、Ohki G、Ishibashi K、Imai M：“单个氨基酸突变导致上皮钙通道快速失活”Biochem Biophys Res Commun。

Ishibashi K, et al.: "Cloning and identification of a new member of water channel (AQP 10) as an aquagly ceroporin"Biochim Biophys Acta. 1576. 335-340 (2002)

Ishibashi K 等人：“水通道新成员 (AQP 10) 作为 aquagly ceroporin 的克隆和鉴定”Biochim Biophys Acta。

Suzuki M, Ooki G, Ishibashi K, Imai M.: "A Single Amino Acid Mutation Results in a Rapid Inactivation of Epithelial Calcium Channels"Biochem Biophys Res Commun.. 291. 278-285 (2002)

Suzuki M、Ooki G、Ishibashi K、Imai M.：“单一氨基酸突变导致上皮钙通道快速失活”Biochem Biophys Res Commun.. 291. 278-285 (2002)