Proliferation mechanisms of endothelial cells through crosstalk between endothelial cells and vascular smooth cells

通过内皮细胞和血管平滑细胞之间的串扰来实现内皮细胞的增殖机制

• 批准号: 11671142
• 负责人: YAMAZAKI Kosuke
• 金额: $ 2.3万
• 依托单位: Asahikawa Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671142/
• 关键词: endothelial cell; vascular smooth muscle cell; transfilter co-culture system; growth factor; apoptosis; survival factor; transfilter co-culture system; 中腹平滑筋細胞

Background: In an animal implantation model, lipo PGE1 administration brought a significant enhancement of Vein graft reendothelialization, and ECs proliferation was enhanced by coculture wth VSMCs. However, the ECs prohferation was not necessarily enhanced by PGE1 in coculture model, whereas endothelial cells (ECs) proliferation was not enhanced by PGE1 in coculture model Our previous study demonstrated ECs proliferation was enhanced by coculture with VSMCs.Objectives: We investigated whether PGE1 prevented apoptotic ECs death.Methods: ECs and VSMCs were harvested from the canine jugular vein, the ECs were cultured in the lower well of a transfilter coculture system, while SMCs were seeded on a transfilter well. When the ECs became confluent, the ECs cultured with the SMCs with daily administration of 50 ng/ml PGE1. Survival rate and apoptosis rate of ECs were measured by a proliferation assay and a fluolescence method with annexin V and Hoechst 33342. The results were compared between the PGE1 group and Control groups (without PGE1 administration) at day 5 and day 7.Result: Survival rate of ECs in the PGEl group and Control group were 92.1 % and 76.0 % at day 1.2 % and 4.7 % at day 7 (P<0.01). Apoptosis rate of ECs were 2,1% and 5.1 % at day 5, and 1.2 % and 4.7 % at day 7, respectively (P<0.01).Conclusion: The present results suggested that PGE1 inhibits apoptotic ECs death, resulting in improvement of ECs Survival rate.

背景资料：在动物移植模型中，给予前列腺素E_1脂微球可显著促进移植静脉的再内皮化，与血管平滑肌细胞共培养可促进内皮细胞增殖。然而，在共培养模型中，PGE 1并不一定促进EC的增殖，而在共培养模型中，PGE 1并不促进内皮细胞（EC）的增殖。从犬颈静脉收获EC和VSMCs，EC在transfilter共培养系统的下孔中培养，而SMCs接种在transfilter孔中。当EC融合时，每天给予50 ng/ml PGE 1，将EC与SMC一起培养。用增殖实验和Annexin V和Hoechst 33342荧光法检测内皮细胞的存活率和凋亡率。结果：PGE 1组和对照组内皮细胞存活率分别为92.1%和76.0%（1.2%和4.7%）（P<0.01）;第5天和第7天的内皮细胞凋亡率分别为2.1%和5.1%和1.2%和4.7%（P<0.01）。