The research of Electrochemotherapy and bone electroconductivity of the mirine osteosarcoma model of bone lesion.

小鼠骨肉瘤骨病变模型的电化学治疗及骨电导率研究。

• 批准号: 11671415
• 负责人: ISHIKAWA Akira
• 金额: $ 1.66万
• 依托单位: YAMAGATA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671415/
• 关键词: osteosarcoma; in vivo electrochemotherapy; electroconductivity of bone; bleomycin; nude rat; drug concentration; histology; tomor necrosis; 骨肉腫; 骨電気伝導性; ヌードラット; bleomyciu; 腫瘍組織内濃度; 腫瘍壊死率; electrochemotherapy; SCIDマウス

INTRODUCTIONElectrochemotherapy is available to enhance the entry of anti-neoplastic agents into murine osteosarcoma cells in the bone lesion. In this experiment model, we investigated the efficacy and cause of electrochemotherapy whether it attributed to the electroconductivity of bone, the distance between electrode and the difference between configuration and number of electrode.MATERIALS AND METHODSTo produce solid murine tumors in bone, resected human osteosarcoma was cut into fine pieces, and inserted into the bone marrow of the hind-limb of Nude rat converting from the SCID mouse.After transplantation, the tumor diameter was checked by X-ray photogram and the experiment was started when it reached 5 mm. The electroporation was done by the electroporator (T820) of BTX.The changes of tumor volume were compared sequentially among the two groups : Drug (D) + Electroporation (E) and neither D nor E.As an anti-neoplastic agent, we used bleomycin (BLM).The electrical resistivity betwee … More n electrodes which held the tumor was measured, and heat (Calaory) which arises by electroporation was calculated.The tumor tissue concentration of BLM was analyzed based on the bio-assay and compared among the two groups : D+E and D without E.Histology was investigated on the tumor necrosis.RESULTS AND DISCUSSIONSThere was the reduction of the tumor diameter in the electroporation (E) group. The reduction of the tumor diameter has been obtained in the group in which the distance between electrodes spread 10mm, 15mm, 20mm and interval. Effective examples were the 23 limbs in 38 limbs.The effective example increased in multiple dose group under operation, when it was compared at the frequency in which 1 time operation, 2 time operation, 3 time operation did (D+E+).The concentration in the tumor tissue of BLM showed the high value, since it has it, in comparing without electroporation group (D+E-) in anti-cancer drug and electroporation group (D+E+), and it seemed to be the effect of electroporation that BLM was incorporated in the interosseous tumor tissue. The production heat calculated from electrical resistivity between tumors did not bring about the effect in anti-tumor effect. In-the example of showing the progress that the bone destruction was restored in X-ray photogram, there were necrosis and elimination of the tumor cell in the pathohistological retrieval. It seemed to be the opinion which proved that electrochemotherapy for the interosseous lesion through the bone has the cytotoxicity reaction.The significance could not be accepted between distance and effect between electrodes. Less

前言电化学疗法可增强抗肿瘤药物进入骨病变中的小鼠骨肉瘤细胞。在这个实验模型中，我们研究了电化学疗法的效果和原因，是否归因于骨的导电性，电极之间的距离和电极的结构和数量的差异。材料和方法为了在骨中产生实体小鼠肿瘤，将切除的人骨肉瘤切成细块，并将其插入由SCID小鼠转化而来的裸鼠的后肢骨髓中。移植后，通过X射线照相检查肿瘤直径，当肿瘤直径达到5 mm时开始实验。比较药物（D）+电穿孔（E）组和药物（D）+电穿孔（E）组肿瘤体积的变化。 ...更多信息 结果与结论电穿孔组（E组）肿瘤直径缩小，而电穿孔组（D + E组）肿瘤直径缩小，BLM组（D+E组）肿瘤直径缩小，BLM组（D+ E组）肿瘤直径缩小，BLM组（D+E组）肿瘤直径缩小，BLM组（D + E组）肿瘤直径缩小。电极间距分别为10 mm、15 mm、20 mm及间隔组瘤体直径均有所缩小。38条患肢中有23条患肢有效，手术中多次给药组与1次手术、2次手术、3次手术次数（D+E+）比较，有效例数增加，BLM在肿瘤组织中的浓度显示高值，因为它有，与未电穿孔组（D+E-）和电穿孔组（D+E+）比较，BLM在骨间肿瘤组织中的掺入似乎是电穿孔的作用。由肿瘤间电阻率计算的产热对抗肿瘤作用无影响。X线片显示骨破坏修复进展的病例，病理组织学检查可见肿瘤细胞坏死、消失。骨间病变经骨电化学治疗有细胞毒性反应的观点似乎是一致的，电极间距离与疗效之间的关系并不显著。少

项目成果

AKIRA ISHIKAWA: "Electrochemotherapy to the Bone Lesion of Murine Osteosarcoma"The Journal of the Japanese Orthopaedic Association. Vol.73. S1227 (1999)

AKIRA ISHIKAWA：“电化学疗法治疗小鼠骨肉瘤骨病变”日本骨科协会杂志。

AKIRA ISHIKAWA: "Low-Voltage Electrochemotherapy to Murine Osteosarcoma Model"The Journal of the Japanese Orthopaedic Association. Vol.74. S1252 (2000)

AKIRA ISHIKAWA：“低电压电化学疗法对小鼠骨肉瘤模型”日本骨科协会杂志。

石川 朗: "マウス骨肉腫の骨内病変に対するElectrochemotherapyの有効性"日本整形外科学会雑誌. 73. S1227 (1999)

Akira Ishikawa：“电化学疗法对小鼠骨肉瘤骨内病变的疗效”日本骨科学会杂志 73. S1227 (1999)。

Ishikawa A: "Electrochemotherapy effect of bleomycin to the murine osteosarcoma."Sosiete International de Recherche en Orthopedie et Traumatologie(SIROT). 8. 733-736 (1999)

Ishikawa A：“博来霉素对小鼠骨肉瘤的电化学治疗效果。”Sosiete International de Recherche en Orthopedie et Traumatologie (SIROT)。

石川 朗: "マウス骨肉腫に対する低電圧Electrochemotherapyの有効性"日本整形外科学会雑誌. 74. S1252 (2000)

Akira Ishikawa：“低压电化学疗法对小鼠骨肉瘤的疗效”，日本骨科学会杂志 74. S1252 (2000)。