Analysis of candidate genes for heterotic QTL discovered from a gene pool of wild mice

100多年前就发现了杂种优势。从那时起，它已成为动植物育种必不可少的遗传现象。然而，尚未克隆出杂种优势的致病基因。我之前从野生小鼠的基因库中发现了一个新的体重杂种优势的 QTL（数量性状位点），并通过使用同源小鼠品系的 QTL 分析将其精细定位到有限的染色体区域。在这项研究中，我使用先前和当前研究中开发的同源和亚同源小鼠品系进行了外显子组分析、表型分析和基因表达分析。我将体重 QTL 精细映射到大约 6 Mb 的基因组区域，但不幸的是，其杂种优势效应在本研究中并未得到证实。我还揭示了四个基因是体重 QTL 的推定候选基因。

Heterosis was found more than 100 years ago. Since then, it has become a genetic phenomenon essential for animal and plant breeding. However, no causative gene for heterosis has been cloned. I previously discovered a new QTL (quantitative trait locus) for heterosis of body weight from a gene pool of wild mice and I fine-mapped it to a limited chromosomal region by QTL analysis using a congenic mouse strain. In this study, I performed exome analysis, phenotypic analysis and gene-expression analysis using congenic and subcongenic mouse strains developed in previous and present studies. I fine-mapped the body weight QTL to an about 6-Mb genomic region, although its heterotic effect was unfortunately not confirmed in this study. I also revealed that four genes are putative candidate genes for the body weight QTL.